BAG3 Antibody


Western Blot: BAG3 Antibody [NBP2-27398] - Analysis of BAG3 using NBP2-27398 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at more
Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP2-27398] - Antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-27398] - Staining of human pancreas tissue sections using this antibody at 1:2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398] - Frozen mouse muscle tissue section stained for BAG-3 expression using NBP2-27398 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to more
Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

BAG3 Antibody Summary

A recombinant protein fragment corresponding to the C-terminal 196 amino acids of human BAG-3. Bag-3; full length-gel=GST-RP; human
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
50 ul neat serum.
0.05% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
1. Increased BAG-3 expression has been described in pancretic cancer compared to normal pancreas, Detectible BAG-3 expression has also been described inserveral pancreatric cancer cell lines. Please see Liao et al (2001) for details.2. In normal organs, BAG-3 protein has been found to be abundantly expressed in muscle. Please see Homma et al for details.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using
NBP2-27398 in the following applications:

  • 2 publications
  • WB
    2 publications

Alternate Names for BAG3 Antibody

  • BAG family molecular chaperone regulator 3
  • BAG-3
  • BAG-family molecular chaperone regulator-3
  • Bcl-2-associated athanogene 3
  • BCL2-associated athanogene 3
  • BCL2-binding athanogene 3
  • Bcl-2-binding protein Bis
  • BIS
  • CAIR-1
  • Docking protein CAIR-1
  • MGC104307


The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S, BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). IMG-5668 recognizes both BAG-3; BAG-3 migrates at ~74-80 kDa on SDS-PAGE.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAG3 Antibody (NBP2-27398)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Gene Symbol BAG3

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