BAG3 Antibody

Images

 
Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Biological Strategies: Immunohistochemistry: BAG3 Antibody [NBP2-27398] - IHC of BAG3 in IR myocardium. BAG3 expression is significantly decreased in the STZ group than those in the CON group. BAG3 expression is ...read more
Biological Strategies: Western Blot: BAG3 Antibody [NBP2-27398] - Western blot of BAG3 in IR myocardium. BAG3 expression is significantly decreased in the STZ group than those in the CON group. BAG3 expression is ...read more
Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP2-27398] - Antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Western Blot: BAG3 Antibody [NBP2-27398] - Analysis of BAG3 using NBP2-27398 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at ...read more
Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398] - Frozen mouse muscle tissue section stained for BAG-3 expression using NBP2-27398 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

BAG3 Antibody Summary

Immunogen
A recombinant protein fragment corresponding to the C-terminal 196 amino acids of human BAG-3. Bag-3
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAG3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000~
  • Immunoprecipitation 1:50-1:200
  • Simple Western 1:100
  • Western Blot 1:1000-1:2000~
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publications using
NBP2-27398 in the following applications:

  • 3 publications
  • WB
    7 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32132902).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50 ul neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for BAG3 Antibody

  • BAG family molecular chaperone regulator 3
  • BAG-3
  • BAG-family molecular chaperone regulator-3
  • Bcl-2-associated athanogene 3
  • BCL2-associated athanogene 3
  • BCL2-binding athanogene 3
  • Bcl-2-binding protein Bis
  • BIS
  • CAIR-1
  • Docking protein CAIR-1
  • MGC104307

Background

The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S , BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). Increased BAG-3 expression has been described in pancretic cancer compared to normal pancreas, Detectible BAG-3 expression has also been described inserveral pancreatric cancer cell lines. Please see Liao et al (2001) for details. In normal organs, BAG-3 protein has been found to be abundantly expressed in muscle. Please see Homma et al for details. The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). IMG-5668 recognizes both BAG-3; BAG-3 migrates at ~74-80 kDa on SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAG3 Antibody (NBP2-27398)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(3)
WB
(7)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-27398 Applications Species
Damiani V, Cufaro MC, Fucito M et al. Proteomics Approach Highlights Early Changes in Human Fibroblasts-Pancreatic Ductal Adenocarcinoma Cells Crosstalk Cells Mar 29 2022 [PMID: 35406724] (WB, Human) WB Human
Yapa Abeywardana M, Samarasinghe KTG, Munkanatta Godage D, Ahn YH Identification and Quantification of Glutathionylated Cysteines under Ischemic Stress Journal of proteome research Aug 12 2021 [PMID: 34382403]
Li X, Lin G, Liu T Et al. Postnatal development of BAG3 expression in mouse cerebral cortex and hippocampus Brain structure & function Aug 6 2021 [PMID: 34357438]
Mukherjee, T, Ramaglia, V Et al. The eIF2 alpha kinase HRI triggers the autophagic clearance of cytosolic protein aggregates. J Biol Chem Dec 2 2020 [PMID: 33410405] (WB, Mouse) WB Mouse
Chien CY, Wen TJ, Cheng YH et al. Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats Antioxidants (Basel) Jul 29 2020 [PMID: 32751309] (IHC-P, WB, Rat) IHC-P, WB Rat
Ormeno F, Hormazabal J, Moreno J et Al. Chaperone Mediated Autophagy Degrades TDP-43 Protein and Is Affected by TDP-43 Aggregation Front Mol Neurosci Feb 18 2020 [PMID: 32132902] (WB, Human, Rat) WB Human, Rat
Li X, Lu J, Kan Q et al. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells Sci Rep 2017 Jul 11 [PMID: 28698547] (WB, Human) WB Human
Aust S, Pils S, Polterauer S et al. Expression of Bcl-2 and the Antiapoptotic BAG Family Proteins in Ovarian Cancer. Appl Immunohistochem Mol Morphol. 2013 Feb 28 [PMID: 23455184] (IHC-P, Human)

Details:
IHC (P): human ovarian cancer tissue microarray (Fig 1). IMGENEX antibodies cited:1. BAG-3 (IMG-5676)2. BAG-4 (IMG-5677).
IHC-P Human
Zhang Y, Wang JH, Lu Q, Wang YJ. Bag3 promotes resistance to apoptosis through Bcl-2 family members in non-small cell lung cancer. Oncol Rep. 2012 Jan [PMID: 21971866]
Goldman A, Chen HD, Roesly HB et al. Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis. Am J Physiol Gastrointest Liver Physiol. 2011 Feb [PMID: 21127259] (WB, Human)

Details:
BAG-3 (IMG-5676). WB: Normal human esophageal epithelial cell lines (HET1A & HET1AR), Fig 4D.
WB Human
Show All 11 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BAG3
Uniprot