Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Biological Strategies: Immunohistochemistry: BAG3 Antibody [NBP2-27398] - IHC of BAG3 in IR myocardium. BAG3 expression is significantly decreased in the STZ group than those in the CON group. BAG3 expression is ...read more
Biological Strategies: Western Blot: BAG3 Antibody [NBP2-27398] - Western blot of BAG3 in IR myocardium. BAG3 expression is significantly decreased in the STZ group than those in the CON group. BAG3 expression is ...read more
Immunocytochemistry/ Immunofluorescence: BAG3 Antibody [NBP2-27398] - Antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Western Blot: BAG3 Antibody [NBP2-27398] - Analysis of BAG3 using NBP2-27398 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at ...read more
Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398] - Frozen mouse muscle tissue section stained for BAG-3 expression using NBP2-27398 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to ...read more
Hu, Mu, RtSpecies Glossary
WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Use in Rat reported in scientific literature (PMID:32132902).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
50 ul neat whole antisera
0.05% Sodium Azide
Alternate Names for BAG3 Antibody
BAG family molecular chaperone regulator 3
BAG-family molecular chaperone regulator-3
Bcl-2-associated athanogene 3
BCL2-associated athanogene 3
BCL2-binding athanogene 3
Bcl-2-binding protein Bis
Docking protein CAIR-1
The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S , BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). Increased BAG-3 expression has been described in pancretic cancer compared to normal pancreas, Detectible BAG-3 expression has also been described inserveral pancreatric cancer cell lines. Please see Liao et al (2001) for details. In normal organs, BAG-3 protein has been found to be abundantly expressed in muscle. Please see Homma et al for details. The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). IMG-5668 recognizes both BAG-3; BAG-3 migrates at ~74-80 kDa on SDS-PAGE.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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