Western Blot: ZAP70 Antibody [NBP1-87000] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: ...read more
Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining of human lymph node shows high expression.
Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining of human cerebral cortex shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining in human lymph node and cerebral cortex tissues using anti-ZAP70 antibody. Corresponding ZAP70 RNA-seq data are ...read more
This antibody was developed against Recombinant Protein corresponding to amino acids: LIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMR
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (85%)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for ZAP70 Antibody
70 kDa zeta-associated protein
Syk-related tyrosine kinase
tyrosine-protein kinase ZAP-70
zeta-chain (TCR) associated protein kinase (70 kD)
zeta-chain (TCR) associated protein kinase 70kDa
zeta-chain associated protein kinase, 70kD
Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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