XRCC1 Antibody (OTI2F8)

Western Blot: XRCC1 Antibody (2F8) [NBP2-02280] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.

Immunocytochemistry/Immunofluorescence: XRCC1 Antibody (2F8) [NBP2-02280] Staining of COS7 cells transiently transfected by pCMV6-ENTRY XRCC1.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - staining of paraffin-embedded bladder tissue using anti-RC204952 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-XRCC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-XRCC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded colon tissue using anti-XRCC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded Kidney tissue using anti-XRCC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded lung tissue using anti-XRCC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded pancreas tissue using anti-XRCC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded prostate tissue using anti-XRCC1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI2F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.64 mg/ml

XRCC1 Antibody (OTI2F8) Summary

• Immunogen: Full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: XRCC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 69.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.64 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XRCC1 Antibody (OTI2F8)

• DNA repair protein XRCC1
• RCC
• X-ray repair complementing defective repair in Chinese hamster cells 1
• X-ray repair cross-complementing protein 1
• X-ray-repair, complementing defective, repair in Chinese hamster

Background

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XRCC1
• Entrez:
  • 7515(Human)