Biological Strategies: Western Blot: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22261 used for WB at 0.1 ug/ml. ...read more
Immunoprecipitation: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22261 used for IP at 6 ug/mg lysate. EXOSC3 was also immunoprecipitated by ...read more
1. After Western blotting, membrane was blocked for 30 minutes in 5% milk dissolved in PBS containing 0.1% Tween 20 (PBST).2. Membrane was blotted with primary antibody diluted in PBST at 4C overnight.
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