DNA Polymerase Kappa Antibody (6F2)

Images

 
Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - POLK monoclonal antibody (M01), clone 6F2 Analysis of POLK expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - analysis of DNA Polymerase Kappa in U87MG (lane 1), T98G (lane 2), A172 (lane 3) and normal human astrocyte (lane 4) lystes using anti-DNA Polymerase ...read more
Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected ...read more
Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.Lane 1: POLK transfected lysate(54.1 KDa).Lane ...read more
ELISA: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, RNAi
Clone
6F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DNA Polymerase Kappa Antibody (6F2) Summary

Immunogen
POLK (AAH50718, 1 a.a. ~ 472 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP
Specificity
POLK - polymerase (DNA directed) kappa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POLK
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • RNA Inhibition
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.
Reviewed Applications
Read 1 Review rated 4
using
H00051426-M01 in the following applications:

Publications
Read Publications using
H00051426-M01 in the following applications:

  • WB
    1 publication

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNA Polymerase Kappa Antibody (6F2)

  • DINB protein
  • DINPPOLQDINB1EC 2.7.7.7
  • DNA polymerase kappa
  • polymerase (DNA directed) kappa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA Polymerase Kappa Antibody (H00051426-M01)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
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Review for DNA Polymerase Kappa Antibody (H00051426-M01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00051426-M01:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot DNA Polymerase Kappa H00051426-M01
Enlarge
4
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedT98G, A172, U87MG and normal human astrocyte lysates
SpeciesHuman
Lot11207-6F2

Blocking

Blocking Details5% (v/v) non-fat milk in phosphate buffered saline and 0.1% (v/v) tween-20, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1 to 5,000, 2 hours, room temperature in 0.1 % (w/v) bovine serum albumin in phosphate buffered saline and 0.1% (v/v) tween-20

Secondary Antibody

Secondary Descriptionstabilized peroxidase conjugated goat anti-mouse (H+L)
Secondary Manufacturer Cat#Thermo Scientific Product# 32430
Secondary Concentration1 to 5,000 dilution

Details

Detection NotesClarity Western chemiluminescence kit (Bio-Rad), visualized using ImageQuant LAS 4000 (GE Healthcare)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNA Polymerase Kappa Antibody (H00051426-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Anonymous
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol POLK
Entrez
OMIM
Uniprot