DNA Polymerase Kappa Antibody (6F2)

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - POLK monoclonal antibody (M01), clone 6F2 Analysis of POLK expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - analysis of DNA Polymerase Kappa in U87MG (lane 1), T98G (lane 2), A172 (lane 3) and normal human astrocyte (lane 4) lystes using anti-DNA Polymerase Kappa antibody. Each lane loaded with 10ug of samples. The primary antibody was used at a dilution of 1:5000 and incubated for 2 hours at room temperature in 0.1 % (w/v) BSA in PBS and 0.1% (v/v) tween-20. Image from verified customer review.

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # H00051426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.Lane 1: POLK transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.

ELISA: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clone: 6F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DNA Polymerase Kappa Antibody (6F2) Summary

• Immunogen: POLK (AAH50718, 1 a.a. ~ 472 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP
• Specificity: POLK - polymerase (DNA directed) kappa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DNA Polymerase Kappa Antibody (6F2)

• DINB protein
• DINPPOLQDINB1EC 2.7.7.7
• DNA polymerase kappa
• polymerase (DNA directed) kappa

Background

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Polymerase Kappa Antibody (H00051426-M01)(2)

Publications using H00051426-M01

• Bostian April C L, Maddukuri Leena, Reed Megan R et al. Kynurenine Signaling Increases DNA Polymerase Kappa Expression and Promotes Genomic Instability in Glioblastoma Cells. Chemical Research in Toxicology 2016-01-01 [PMID: 26651356] (WB, Human)
• Sekimoto T, Oda T, Kurashima K et al. Both High-fidelity Replicative and Low-fidelity Y-family Polymerases are Involved in DNA Rereplication. Mol Cell Biol. 2014-12-08 [PMID: 25487575]

Review for DNA Polymerase Kappa Antibody (H00051426-M01) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Robert Eoff WB Human 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: T98G, A172, U87MG and normal human astrocyte lysates
• Species: Human
• Lot: 11207-6F2

Bioinformatics

• Gene Symbol: POLK
• Entrez:
  • 51426(Human)
• OMIM:
  • 605650(Human)
• Uniprot:
  • AAH50718(Human)