XPA Antibody Summary
Immunogen |
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human xeroderma pigmentosum, complementation group A using the numbering given in entry NP_000371.1 (GeneID 7507). |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
XPA |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 1:200 - 1:1000
- Immunohistochemistry-Paraffin 1:200 - 1:1000
- Immunoprecipitation 2 - 10 ug/mg lysate
- Western Blot 1:2000-1:10000
|
Application Notes |
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Control |
|
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
TBS and 0.1% BSA |
Preservative |
0.09% Sodium Azide |
Concentration |
0.2 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for XPA Antibody
Background
This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for XPA Antibody (NB100-93321) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for XPA Antibody (NB100-93321). (Showing 1 - 1 of 1 FAQ).
-
What's the size of protein that your XPA antibody (NB100-93321) detects?
- Xeroderma pigmentosum group A-complementing protein (XPA) has an expected molecular weight of approximately 31 kD and this protein undergoes post-translational modifications (PTMs) such as phosphorylation as well as ubiquitination that leads to small increase in its observed molecular weight in Western blots. Our XPA antibody (NB100-93321) has been validated in whole cell lysate from HeLa cells, wherein it detected specific single band at 39kDa.