Role of RASSF1A in Death Receptor-Dependent Apoptosis

Wed, 07/14/2010 - 07:55

Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and therefore have a large antibody database targeting death receptor proteins.

RASSF1A (Ras association domain family protein 1A) is a tumor-suppressor gene known to play a key role in death-receptor apoptosis. It is expressed in a range of tissues, among them kidney, pancreas, spleen, thymus, brain, lung, liver, prostate, testis and peripheral blood leukocyte cells. Inhibition or mutation of this gene is linked to a variety of cancers.

RASSF1A expression is inhibited when CpG islands (areas where there are high numbers of cytosine-phosphate-guanine or CpG sites) are hypermethylated. Hypermethylation of the RASSF1A promoter is linked to the development of various tumors. Encoded protein is known to induce cell cycle arrest by inhibiting cyclin D1, and it also interacts with XPA, a DNA repair protein. However, the activation process is unclear.

Western Blot: RASSF1 Antibody Western Blot: RASSF1 Antibody

Recently, Ghazaleh, et al. shed new light on its mechanism of action, demonstrating the important role of 14-3-3 proteins in regulating RASSF1A-initiated apoptosis.14-3-3 (so named because of its migration patterns in antibody assays) is a regulatory family of proteins, which binds to a wide range of signaling proteins.

The study showed that stimulation with the tumor necrosis factor TNF alpha, or TNF alpha related apoptosis inducing ligand (TRAIL) blocked the RASSF1A/14-3-3 binding process, following which RASSF1A bonded to MAP1 (Modulator of Apoptosis-1). 14-3-3 bonding required basal phosphorylation by GSK3 beta at three critical serine sites. Antibody assays also revealed that mutation of these serines led to loss of 14-3-3 association and subsequent recruitment to MAP1.

Novus Biologicals offers many RASSF1A reagents for your research needs including:

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