XIRP1 [p Ser295] Antibody - Azide Free

Western Blot: XIRP1 [p Ser 295] Antibody [NBP3-11953] - Western blot of mouse heart lysate showing specific immunolabeling of the ~250 kDa and ~150 kDa splice variants of the XIRP1 protein phosphorylated at Ser295 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 minutes).

• Reactivity: Mu, Hu, Rt, Ca, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

XIRP1 [p Ser295] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit XIRP1 [p Ser295] Antibody - Azide Free (NBP3-11953) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 conjugated to KLH. (UniProt: O70373)
• Modification: p Ser295
• Specificity: Specific for endogenous levels of the ~250 kDa and ~150 kDa splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Rat (100%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XIRP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for XIRP1 [p Ser295] Antibody - Azide Free

• cardiomyopathy associated 1
• CMYA1
• DKFZp779C1255
• xin actin-binding repeat containing 1

Background

Protects actin filaments from depolymerization

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XIRP1