Orthogonal Strategies: Dual RNAscope ISH-IHC: xCT Antibody Pack [NB100-937] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for xCT mRNA (ACD RNAScope Probe, catalog #422688; Fast ...read more
Simple Western: xCT Antibody Pack [NB100-937] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was performed under reducing ...read more
Western Blot: xCT Antibody Pack [NB100-937] - Detection of XCT in total human and mouse stomach lysate, respectively, using NB 300-318. 1 minute ECL exposure.
Immunohistochemistry: xCT Antibody Pack [NB100-937] - xCT expression is enhanced in CD68+ cells from MS spinal cord. CD68+ cells (arrows) show enhanced xCT expression in MS patients as compared to controls. CD68+ ...read more
Western Blot: xCT Antibody Pack [NB100-937] - Total protein from human HeLa, Huvec, HCT-116 and A549 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Western Blot: xCT Antibody Pack [NB100-937] - Detection of XCT in total human stomach lysate using NB 300-317. 15 minute ECL exposure.
This pack contains 1 vial each of: NB300-317 (0.1 mL) and NB300-318 (0.1 mL).
Alternate Names for xCT Antibody Pack
Amino acid transport system xc-
Calcium channel blocker resistance protein CCBR1
solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
Solute carrier family 7 member 11
solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. Mammalian amino acid transport systems consist of a large variety of transporters. The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane. System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates. The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc. In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress. Recent studies have implicated xCT as a gateway that Kaposi's sarcoma herpesvirus (KSHV) uses to enter cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
FAQs for xCT Antibody Pack (NB100-937). (Showing 1 - 1 of 1 FAQ).
Do you have a slc7a11 antibody that is conjugated, reacts to mouse, works with cell surface staining, does not need permeabilization?
SLC7A11 or xCT antibody NB300-318AF594 targets a cytoplasmic region of the protein, so it should work without permeabilization. However, the data does say it was permeabilized with saponin, so we cannot say for certain if it will work without permeabilization.
xCT: The Membrane's Gatekeeper xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially... Read full blog post.
xCT: Friend or Foe? There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has... Read full blog post.
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