XCL1/Lymphotactin Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

XCL1/Lymphotactin Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human XCL1/Lymphotactin (R&D Systems, Catalog # 695-LT)
  Val22-Gly114
  Accession # P47992
• Specificity: Detects human XCL1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.15% cross-reactivity with recombinant mouse XCL1, recombinant human (rh) Eotaxin, and rhEotaxin-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: XCL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human XCL1/Lymphotactin Antibody (Catalog # MAB6951)
  ELISA Detection: Human XCL1/Lymphotactin Biotinylated Antibody (Catalog # BAF695)
  Standard: Recombinant Human XCL1/Lymphotactin (Catalog # 695-LT)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XCL1/Lymphotactin Antibody [Biotin]

• ATAC
• ATACSmall-inducible cytokine C1
• C motif chemokine 1
• chemokine (C motif) ligand 1
• Cytokine SCM-1
• LPTNSCM-1-alpha
• LTNXC chemokine ligand 1
• Lymphotactin
• Lymphotaxin
• SCM-1 alpha
• SCM-1
• SCM1A
• SCM-1a
• SCYC1SCM1
• single cysteine motif 1a
• small inducible cytokine subfamily C, member 1 (lymphotactin)
• XCL1

Background

Human lymphotactin (Lptn)/XCL1 (also named human SCM-1 alpha and ATAC) and its mouse homologue belong to the C or gamma  subfamily of chemokines. The C chemokines lack two (the 1st and 3rd ) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Human lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is abundant in some activated T cells such as activated CD8⁺ T cells and other class I MHC restricted T cells. Lptn expression is absent in CD4⁺ T cells. Human and mouse Lptn share approximately 60% amino acid sequence homology. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant human lymphotactin has been shown to have chemotactic activity for lymphocytes and NK cells. The orphan receptor GPR5 has been reported to be the specific receptor for Lptn.

1. Kennedy, J. et al. (1995) J. Immunol. 155:203.
2. Zlotnik, A. et al. (1999) Critical Reviews in Immunol. 19:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XCL1/Lymphotactin Antibody (BAF695)(1)

Publication using BAF695

• Chen DS, Soen Y, Stuge TB Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray. PLoS Med., 2005-09-20;2(10):e265. 2005-09-20 [PMID: 16162034] (Functional Assay, Human)

Bioinformatics

• Gene Symbol: XCL1
• Uniprot:
  • P47992()