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486-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse XCL1/Lymphotactin (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486). The ND50 is typically 5-15 µg/mL." class="big_lightbox" target="_blank">
486-LT) was serially diluted and captured by Rat Anti-Mouse XCL1/Lymphotactin Monoclonal Antibody (Catalog # MAB486) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse XCL1/Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
695-LT) was serially diluted and captured by Mouse Anti-Human XCL1/Lymphotactin Monoclonal Antibody (Catalog # MAB6951) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human XCL1/Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF695) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
486-LT) was serially diluted and captured by Rat Anti-Mouse XCL1/Lymphotactin Monoclonal Antibody (Catalog # MAB486) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse XCL1/Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">