XCL1/Lymphotactin Antibody (109001)

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 109001
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

XCL1/Lymphotactin Antibody (109001) Summary

• Immunogen: E. coli-derived recombinant human XCL1/Lymphotactin
  Val22-Gly114
  Accession # P47992
• Specificity: Detects human XCL1/Lymphotactin in ELISAs and Western blots. In sandwich ELISAs, less than 0.15% cross-reactivity was observed with recombinant mouse MCP‑5, recombinant human (rh) MIP‑3 beta, rhGRO alpha, rhTGF‑ alpha, rhGRO gamma, rhActivin RII Fc Chimera, rhIGF‑II, or rhIL‑15.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: XCL1
• Purity: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human XCL1/Lymphotactin Biotinylated Antibody (Catalog number BAF695)
  Standard: Recombinant Human XCL1/Lymphotactin (Catalog number 695-LT)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XCL1/Lymphotactin Antibody (109001)

• ATAC
• ATACSmall-inducible cytokine C1
• C motif chemokine 1
• chemokine (C motif) ligand 1
• Cytokine SCM-1
• LPTNSCM-1-alpha
• LTNXC chemokine ligand 1
• Lymphotactin
• lymphotaxin
• SCM-1 alpha
• SCM-1
• SCM1A
• SCM-1a
• SCYC1SCM1
• single cysteine motif 1a
• small inducible cytokine subfamily C, member 1 (lymphotactin)
• XCL1

Background

Human lymphotactin (Lptn)/XCL1 (also named human SCM-1 alpha and ATAC) and its mouse homologue belong to the C or gamma  subfamily of chemokines. The C chemokines lack two (the 1st and 3rd ) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Human lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is abundant in some activated T cells such as activated CD8⁺ T cells and other class I MHC restricted T cells. Lptn expression is absent in CD4⁺ T cells. Human and mouse Lptn share approximately 60% amino acid sequence homology. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant human lymphotactin has been shown to have chemotactic activity for lymphocytes and NK cells. The orphan receptor GPR5 has been reported to be the specific receptor for Lptn.

1. Kennedy, J. et al. (1995) J. Immunol. 155:203.
2. Zlotnik, A. et al. (1999) Critical Reviews in Immunol. 19:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XCL1/Lymphotactin Antibody (MAB6951)(3)

Publications using MAB6951

• A Gudjonsson, A Lysén, S Balan, V Sundvold-G, C Arnold-Sch, L Richter, ES Bækkevold, M Dalod, B Bogen, E Fossum Targeting Influenza Virus Hemagglutinin to Xcr1(+) Dendritic Cells in the Absence of Receptor-Mediated Endocytosis Enhances Protective Antibody Responses J. Immunol, 2017;0(0):. 2017 [PMID: 28228559] (Neutralization, Human)
• Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1. PLoS Pathog, 2013;9(12):e1003852. 2013 [PMID: 24385911] (Capture Assay, Virus - HIV-1)
• Cao W, Tang S, Yuan H, Wang H, Zhao X, Lu H Mycobacterium tuberculosis antigen Wag31 induces expression of C-chemokine XCL2 in macrophages. Curr. Microbiol., 2008;57(3):189-94. 2008 [PMID: 18618175] (Western Blot, Human)

Bioinformatics

• Gene Symbol: XCL1
• Uniprot:
  • P47992()