WWOX Antibody (SR2547)

Immunohistochemistry-Paraffin: WWOX Antibody (SR2547) [NBP3-27813] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western Blot: WWOX Antibody (SR2547) [NBP3-27813] - Western blot analysis of WWOX in Hela, U2OS, U251MG lysates

Western Blot: WWOX Antibody (SR2547) [NBP3-27813] - Western blot analysis of WWOX in mouse testis, mouse spleen lysates

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clone: SR2547
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

WWOX Antibody (SR2547) Summary

• Description: Novus Biologicals Rabbit WWOX Antibody (SR2547) (NBP3-27813) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human WWOX (Uniprot #: Q9NZC7)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WWOX
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunoprecipitation 1:20
  • Western Blot 1:500-1:1000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for WWOX Antibody (SR2547)

• D16S432E
• EC 1.1.1
• EC 1.1.1.-
• FOR
• FORHHCMA56
• FRA16D
• Fragile site FRA16D oxidoreductase
• SDR41C1
• short chain dehydrogenase/reductase family 41C, member 1
• WOX1
• WOX1PRO0128
• WW domain containing oxidoreductase
• WW domain-containing oxidoreductase
• WW domain-containing protein WWOX
• WWOX

Background

WW domain-containing oxidoreductase (WWOX) is a proapoptotic protein and a tumor suppressor gene. Because mutation or deletion in the coding region of WWOX is rarely found, it is speculated that the appearance of aberrant transcripts affects progression of many cancers. In addition, protein levels of WWOX, its Tyr33-phosphorylated form, and WOX2 are significantly down-regulated in the neurons of Alzheimer's hippocampi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WWOX