SPAM1 Antibody


Western Blot: SPAM1 Antibody [NBP2-93736] - Analysis of extracts of various cell lines, using SPAM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 more
Immunocytochemistry/ Immunofluorescence: SPAM1 Antibody [NBP2-93736] - Analysis of human testis cells using SPAM1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP2-93736] - Rat testis using SPAM1 Rabbit pAb at dilution of 1:100 (40x lens).
Immunocytochemistry/ Immunofluorescence: SPAM1 Antibody [NBP2-93736] - Analysis of rat testis cells using SPAM1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P

Order Details

SPAM1 Antibody Summary

Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).
Affinity purified
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  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.3), 50% glycerol
0.05% Proclin 300
Affinity purified

Alternate Names for SPAM1 Antibody

  • EC
  • HYA1
  • HYAL1
  • HYAL3
  • HYAL5
  • hyal-PH20
  • hyaluronidase PH-20
  • Hyaluronoglucosaminidase PH-20
  • MGC26532
  • PH20
  • PH-20
  • SPAG15
  • SPAM1
  • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
  • Sperm adhesion molecule 1
  • Sperm surface protein PH-20


Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Funny Protein Names Infographic
This is not a joke, these proteins with funny names actually do exist. View our list of six proteins with funny and unusual names including: Bambi, Yippee-like 3, Wee1, SPAM1, SPOCK1 and Bagpipe homeobox protein homolog 1. Learn more about their fu...  Read full blog post.

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Gene Symbol SPAM1