Frizzled-5 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Frizzled-5 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Frizzled‑5
Ala27-Pro167
Accession # AAC50385
Specificity
Detects human Frizzled-5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FZD5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1617 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-5 Antibody

  • C2orf31
  • chromosome 2 open reading frame 31
  • DKFZP434E2135
  • frizzled (Drosophila) homolog 5
  • frizzled homolog 5 (Drosophila)
  • Frizzled5
  • Frizzled-5
  • Frz-5
  • FZ-5
  • FZD5
  • fzE5
  • HFZ5
  • MGC129692
  • seven-transmembrane receptor frizzled-5
  • Wnt receptor

Background

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled (Frz) proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 amino acid (aa) and contains 10 invariant cysteines (1). Human Frz-5 shows 95% aa identity to mouse Frz-5 in the CRD region. In the mouse, Frz-5 is expressed in adult tissues (heart and kidney), as well as embryonic tissues (telencephalon, eye, and lung bud) (2, 3). Null mutations in Frz-5 reveal that it plays a role in the formation and maintenance of the extra-embryonic vasculature (3). Functional interactions with Frz-5 have been reported for Wnt-5a, Wnt-10b, Wnt-2b, and Wnt-7a, implicating these Wnts as ligands for the Frz-5 receptor (3-5).

Two distinct Wnt signal transduction pathways have been characterized. One is the canonical Wnt/ beta -catenin pathway that is involved in diverse biological mechanisms such as dorsal/ventral development in Xenopus embryos and mammalian tumor formation. Frz-5 is implicated in this pathway based on its ability to induce beta -catenin target genes in the presence of ligand (5). However, Frz-5 is also implicated in beta -catenin independent pathways (4, 6).

  1. Wang, Y. et al. (1996) J. Biol. Chem 271:4468.
  2. Borello, U. et al. (1999) Mech. Dev. 89:173.
  3. Ishikawa, T. et al. (2001) Development 128:25.
  4. He, X. et al. (1997) Science 275:1652.
  5. Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
  6. Veeman, M. et al. (2003) Dev. Cell 5:367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Frizzled-5 Antibody (AF1617)(6)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 5 applications: Bioassay, ELISA Development, Functional Assay, IHC Frozen, WB.


Filter By Application
Bioassay
(1)
ELISA Development
(1)
Functional Assay
(1)
IHC Frozen
(1)
WB
(2)
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Human
(2)
Mouse
(3)
N/A
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF1617 Applications Species
Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation J Biol Chem, 2016;0(0):. 2016 [PMID: 27402827] (Bioassay, N/A) Bioassay N/A
Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE, 2014;9(8):e104937. 2014 [PMID: 25170755] (Functional Assay,, Human) Functional Assay Human
Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity. PLoS ONE, 2011;6(8):e22920. 2011 [PMID: 21857966] (WB,, Mouse) WB Mouse
Kilander MB, Dijksterhuis JP, Ganji RS, Bryja V, Schulte G WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins. Cell. Signal., 2011;23(3):550-4. 2011 [PMID: 21070854] (WB,, Mouse) WB Mouse
Gudjonsson JE, Johnston A, Stoll SW Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 2010;130(7):1849-59. 2010 [PMID: 20376066] (IHC Frozen,, Human) IHC Frozen Human
Magdesian MH, Carvalho MM, Mendes FA, Saraiva LM, Juliano MA, Juliano L, Garcia-Abreu J, Ferreira ST Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling. J. Biol. Chem., 2008;283(14):9359-68. 2008 [PMID: 18234671] (ELISA Development,, Mouse) ELISA Development Mouse

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Bioinformatics

Gene Symbol FZD5
Entrez
Uniprot