Wnt-6 Antibody

Wnt-6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human Wnt-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4190) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-6 Antibody Summary

• Immunogen: E. coli-derived recombinant human Wnt‑6
  Met35-Glu75 and Gln238-Arg299
  Accession # Q9Y6F9
• Specificity: Detects human Wnt-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Wnt-4, rmWnt-8a, and rmWnt-9b is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: WNT6
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-6 Antibody

• protein Wnt-6
• wingless-type MMTV integration site family, member 6
• Wnt6
• Wnt-6

Background

Human Wnt-6 is a 46 kDa, secreted, glycoprotein of the Wnt family. Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region. The mature molecule contains 24 cysteines and one potential N-linked glycosylation site. Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells, and limb bud ectoderm. Over the regions used as immunogen, the aa sequences of human, mouse, and canine Wnt-6 are identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT6
• Uniprot:
  • Q9Y6F9()