Wnt-7b Antibody

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Western blot shows lysates of JAR human choriocarcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt‑7b Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Wnt-7b Antibody Summary

Immunogen
E. coli-derived recombinant human Wnt‑7b
Leu32-Glu72 & Thr216-Ala283
Accession # P56706
Specificity
Detects human Wnt-7b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-7a is observed and less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rhWnt‑9a, rmWnt-11, and rmWnt-16 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WNT7B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF3460 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-7b Antibody

  • protein Wnt-7b
  • wingless-type MMTV integration site family, member 7B
  • Wnt7b
  • Wnt-7b

Background

Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Human Wnt-7b is synthesized as a 349 amino acid (aa) precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Mature human Wnt-7b shares 99% aa sequence identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa sequence identity with Wnt-7a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Wnt-7b Antibody (AF3460)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF3460 Applications Species
M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling Respir. Res, 2017;18(1):52. 2017 [PMID: 28340578] (IHC-Fr, Human) IHC-Fr Human
Bruce F Kimler WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation J. Endocrinol., 2016;229(2):197-207. 2016 [PMID: 26975616] (IHC-P, Rat) IHC-P Rat
Shiah S, Hsiao J, Chang W, Chen Y, Jin Y, Wong T, Huang J, Tsai S, Hsu Y, Chou S, Yen Y, Jiang S, Shieh Y, Chang I, Hsiao M, Chang J Downregulated miR329 and miR410 promote the proliferation and invasion of oral squamous cell carcinoma by targeting Wnt-7b. Cancer Res, 2014;74(24):7560-72. 2014 [PMID: 25351956] (IHC, Human) IHC Human
Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells. Int J Oncol, 2012;41(6):2021-8. 2012 [PMID: 23076981] (Western Blot, Human) Western Blot Human
Volckaert T, Dill E, Campbell A, Tiozzo C, Majka S, Bellusci S, De Langhe SP Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. J. Clin. Invest., 2011;121(11):4409-19. 2011 [PMID: 21985786] (IHC-P, Mouse) IHC-P Mouse
Gogolla N, Galimberti I, Deguchi Y, Caroni P Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus. Neuron, 2009;62(4):510-25. 2009 [PMID: 19477153] (Western Blot, Mouse) Western Blot Mouse

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Product General Protocols

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Bioinformatics

Gene Symbol WNT7B
Uniprot