Western blot shows lysates of JAR human choriocarcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt‑7b Antigen Affinity-purified Polyclonal ...read more
E. coli-derived recombinant human Wnt‑7b Leu32-Glu72 & Thr216-Ala283 Accession # P56706
Specificity
Detects human Wnt-7b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-7a is observed and less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rhWnt‑9a, rmWnt-11, and rmWnt-16 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WNT7B
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Wnt-7b Antibody
protein Wnt-7b
wingless-type MMTV integration site family, member 7B
Wnt7b
Wnt-7b
Background
Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the
Wnt family. Wnt proteins can be lipid-modified and are ligands for
members of the frizzled family of receptors, which mediates cell-cell
communication during development. Human Wnt-7b is synthesized as a 349
amino acid (aa) precursor that contains a 318 aa mature region. The
mature region contains 24 cysteines and three potential N-linked
glycosylation sites. Mature human Wnt-7b shares 99% aa sequence
identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa
sequence identity with Wnt-7a.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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