Recombinant Human/Mouse Wnt-5a Protein


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Product Details

Reactivity Hu, MuSpecies Glossary
Applications Bioactivity

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Recombinant Human/Mouse Wnt-5a Protein Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.
Optimal concentrations should be determined by each laboratory for each application.
Chinese Hamster Ovary cell line, CHO-derived Wnt-5a protein
Accession #
N-terminal Sequence
Asn44 & No results obtained: Gln38 predicted
Protein/Peptide Type
Recombinant Proteins
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
45 kDa, reducing conditions
Read Publications using
645-WN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse Wnt-5a Protein

  • hWNT5A
  • protein Wnt-5a
  • wingless-type MMTV integration site family, member 5A
  • WNT-5A protein
  • Wnt5a
  • Wnt-5a


Wnt-5a is a 44‑50 kDa member of the Wnt family of proteins (1‑6). Based on its activity towards C57Mg mammary epithelium, it is classified as a nontransforming Wnt. Human Wnt‑5a is synthesized as a 380 amino acid (aa) precursor that contains a 37 aa signal sequence, a 25 aa prosegment, and a 319 aa mature region (1, 2, 3). The mature region has 24 cysteine residues that form multiple intrachain disulfide bonds, plus four N‑linked glycosylation sites that are utilized for proper secretion (3, 5, 7). There is also a palmitate adduct at Cys104 that is essential for activity, and a potential palmitoleic acid modification at Ser244 that may also contribute to secretion (7‑9). One alternative start site is reported at Met16. Over aa 38‑380, human and mouse Wnt‑5a are identical in amino acid sequence (1, 10). Cells known to express Wnt‑5a include brainstem astrocytes (11), mammary epithelium (12), CD34+ primitive progenitor stem cells (13), chondrocytes (14), CD34- pericytes and vascular smooth muscle cells (15), plus mesenchymal cells at various sites (16, 17). There are multiple receptors for Wnt‑5a. These include Fzd-1, -2,
-3, -4, -5, and -7 (3, 18‑22), Ror2 (3), LRP6 (23), Ryk (24) and sFRP1 (25). All these molecules function within the context of a larger number of “co‑factors” that regulate signaling by the Wnts. Initially, it was suggested that there were three pathways for Wnt signaling; a beta -catenin-mediated canonical pathway, and two noncanonical pathways described as the Wnt/JNK (PCP) pathway and the Wnt/Ca++ pathway (26, 27). And it was assumed that various Wnts could be accommodated by these classifications. At present, it is now recognized that individual Wnts, through various combinations of receptor complex subunits, can have diverse effects, perhaps even within the same cell (3, 6, 27). Further complexity is introduced by the fact that Xenopus Wnt‑5a and Wnt‑11 are known to form bioactive heterodimers following Tyr sulfation (28). Thus, predicting the activity of Wnt‑5a, or any other Wnt, on any cell type will require substantial insight into the interaction between all the extracellular, cell surface and intracellular components of the Wnt signaling system.

  1. Clark, C.C. et al. (1993) Genomics 18:249.
  2. LeJeune, S. et al. (1995) Clin. Cancer Res. 1:215.
  3. Mikels, A.J. & R. Nusse (2006) PLoS Biol. 4:e115.
  4. Nishita, M. et al. (2010) Trends Cell Biol. 20:346.
  5. Mikels, A.J. & R. Nusse (2006) Oncogene 25:7461.
  6. van Amerongen, R. & R. Nusse (2009) Development 136:3205.
  7. Kurayoshi, M. et al. (2007) Biochem. J. 402:515.
  8. Takada, R. et al. (2006) Dev. Cell 11:791.
  9. Port, F. & K. Basler (2010) Traffic May 3. [Epub ahead of print].
  10. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
  11. Castelo-Branco, G. et al. (2006) Mol. Cell. Neurosci. 31:251.
  12. Jonsson, M. et al. (1998) Br. J. Cancer 78:430.
  13. van Den Berg, D.J. et al. (1998) Blood 92:3189.
  14. Kruger, C. & C. Kappen (2010) PLoS One 5:e8978.
  15. Lin, G. et al. (2008) Stem Cells Dev. 17:1053.
  16. Lickert, H. et al. (2001) Mech. Dev. 105:181.
  17. Danielson, K.G. et al. (1995) J. Biol. Chem. 270:31225.
  18. Gazit, A. et al. (1999) Oncogene 18:5959.
  19. Bazhin, A. V. et al. (2010) Cell. Mol. Life Sci. 67:817.
  20. Kawasaki, A. et al. (2007) Cell. Signal. 19:2498.
  21. Blumenthal, A. et al. (2006) Blood 108:965.
  22. Umbhauer, M. et al. (2000) EMBO J. 19:4944.
  23. Bryja, V. et al. (2009) Mol. Biol. Cell 20:924.
  24. Keeble, T.R. et al. (2006) J. Neurosci. 26:5840.
  25. Lin, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11196.
  26. Rao, T.P. & M. Kuhl (2010) Circ. Res. 106:1798.
  27. McDonald, S.L. & A. Silver (2009) Br. J. Cancer 101:209.
  28. Cha, S-W. et al. (2009) Curr. Biol. 19:1573.

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Publications for Wnt-5a (645-WN)(126)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Zebrafish.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, In Vivo, Western Blot.

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Showing Publications 1 - 10 of 126. Show All 126 Publications.
Publications using 645-WN Applications Species
E Flores-Her, DM Velázquez, MC Castañeda-, G Fuentes-Ga, G Fonseca-Ca, JK Yamamoto-F, MT Romero-Avi, JA García-Sái, M Robles-Flo Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells Cell. Signal., 2020;72(0):109636. 2020 [PMID: 32283254] (Bioassay, Human) Bioassay Human
P Kozielewic, A Turku, CF Bowin, J Petersen, J Valnohova, MCA Cañizal, Y Ono, A Inoue, C Hoffmann, G Schulte Structural insight into small molecule action on Frizzleds Nat Commun, 2020;11(1):414. 2020 [PMID: 31964872] (Cell Culture, Human) Cell Culture Human
C Luo, E Balsa, EA Perry, J Liang, CD Tavares, F Vazquez, HR Widlund, P Puigserver H3K27me3-mediated PGC1&amp;alpha gene silencing promotes melanoma invasion through WNT5A and YAP J. Clin. Invest., 2020;130(2):853-862. 2020 [PMID: 31929186] (Bioassay, Human) Bioassay Human
MM de Rezende, JP Ng-Blichfe, GZ Justo, EJ Paredes-Ga, R Gosens Divergent effects of Wnt5b on IL-3- and GM-CSF-induced myeloid differentiation Cell. Signal., 2019;67(0):109507. 2019 [PMID: 31857239] (Bioassay, Human) Bioassay Human
F Zhao, L Zhou, J Liu, Z Xu, W Ping, H Li, L Xu, Z Xu, C Zhou, M Wang, R Jia Construction of a vascularized bladder with autologous adipose-derived stromal vascular fraction cells combined with bladder acellular matrix via tissue engineering J Tissue Eng, 2019;10(0):2041731419891. 2019 [PMID: 31827758] (Bioassay, Rat) Bioassay Rat
X Wu, EM van Dijk, JP Ng-Blichfe, IST Bos, C Ciminieri, M Königshoff, LEM Kistemaker, R Gosens Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors Cells, 2019;8(10):. 2019 [PMID: 31557955] (Bioassay, Human) Bioassay Human
G Barbero, MV Castro, MB Villanueva, MJ Quezada, NB Fernández, S DeMorrow, P Lopez-Berg An Autocrine Wnt5a Loop Promotes NF-kappaB Pathway Activation and Cytokine/Chemokine Secretion in Melanoma Cells, 2019;8(9):. 2019 [PMID: 31510045] (Bioassay, Human) Bioassay Human
Z Ji, W Zhao, HK Lin, X Zhou Systematically understanding the immunity leading to CRPC progression PLoS Comput. Biol., 2019;15(9):e1007344. 2019 [PMID: 31504033] (Bioassay, Human) Bioassay Human
K Kaiser, D Gyllborg, J Procházka, A Salašová, P Kompaníkov, FL Molina, R Laguna-Goy, T Radaszkiew, J Harnoš, M Procházkov, D Pot?šil, RA Barker, ÁG Casado, Z Zdráhal, R Sedlá?ek, E Arenas, JC Villaescus, V Bryja WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis Nat Commun, 2019;10(1):1498. 2019 [PMID: 30940800] (Bioassay, Mouse) Bioassay Mouse
G Lutze, A Haarmann, JA Demanou To, K Buttler, J Wilting, J Becker Non-canonical WNT-signaling controls differentiation of lymphatics and extension lymphangiogenesis via RAC and JNK signaling Sci Rep, 2019;9(1):4739. 2019 [PMID: 30894622] (Bioassay, Human) Bioassay Human
Show All 126 Publications.

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The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

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Gene Symbol WNT5A