Recombinant Human/Mouse Wnt-5a Protein

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Recombinant mouse Wnt-5a (645-WN) inhibits Wnt-3a-induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED50 for this effect is 0.1-0.5 µg/mL in the presence of 5 ng/mL recombinant ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human/Mouse Wnt-5a Protein Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.
Optimal concentrations should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived Wnt-5a protein
Gln38-Lys380
Accession #
N-terminal Sequence
Asn44 & No results obtained: Gln38 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
WNT5A
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45 kDa, reducing conditions
Publications
Read Publications using
645-WN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse Wnt-5a Protein

  • hWNT5A
  • protein Wnt-5a
  • wingless-type MMTV integration site family, member 5A
  • WNT-5A protein
  • Wnt5a
  • Wnt-5a

Background

Wnt-5a is a 44‑50 kDa member of the Wnt family of proteins (1‑6). Based on its activity towards C57Mg mammary epithelium, it is classified as a nontransforming Wnt. Human Wnt‑5a is synthesized as a 380 amino acid (aa) precursor that contains a 37 aa signal sequence, a 25 aa prosegment, and a 319 aa mature region (1, 2, 3). The mature region has 24 cysteine residues that form multiple intrachain disulfide bonds, plus four N‑linked glycosylation sites that are utilized for proper secretion (3, 5, 7). There is also a palmitate adduct at Cys104 that is essential for activity, and a potential palmitoleic acid modification at Ser244 that may also contribute to secretion (7‑9). One alternative start site is reported at Met16. Over aa 38‑380, human and mouse Wnt‑5a are identical in amino acid sequence (1, 10). Cells known to express Wnt‑5a include brainstem astrocytes (11), mammary epithelium (12), CD34+ primitive progenitor stem cells (13), chondrocytes (14), CD34- pericytes and vascular smooth muscle cells (15), plus mesenchymal cells at various sites (16, 17). There are multiple receptors for Wnt‑5a. These include Fzd-1, -2,
-3, -4, -5, and -7 (3, 18‑22), Ror2 (3), LRP6 (23), Ryk (24) and sFRP1 (25). All these molecules function within the context of a larger number of “co‑factors” that regulate signaling by the Wnts. Initially, it was suggested that there were three pathways for Wnt signaling; a beta -catenin-mediated canonical pathway, and two noncanonical pathways described as the Wnt/JNK (PCP) pathway and the Wnt/Ca++ pathway (26, 27). And it was assumed that various Wnts could be accommodated by these classifications. At present, it is now recognized that individual Wnts, through various combinations of receptor complex subunits, can have diverse effects, perhaps even within the same cell (3, 6, 27). Further complexity is introduced by the fact that Xenopus Wnt‑5a and Wnt‑11 are known to form bioactive heterodimers following Tyr sulfation (28). Thus, predicting the activity of Wnt‑5a, or any other Wnt, on any cell type will require substantial insight into the interaction between all the extracellular, cell surface and intracellular components of the Wnt signaling system.

  1. Clark, C.C. et al. (1993) Genomics 18:249.
  2. LeJeune, S. et al. (1995) Clin. Cancer Res. 1:215.
  3. Mikels, A.J. & R. Nusse (2006) PLoS Biol. 4:e115.
  4. Nishita, M. et al. (2010) Trends Cell Biol. 20:346.
  5. Mikels, A.J. & R. Nusse (2006) Oncogene 25:7461.
  6. van Amerongen, R. & R. Nusse (2009) Development 136:3205.
  7. Kurayoshi, M. et al. (2007) Biochem. J. 402:515.
  8. Takada, R. et al. (2006) Dev. Cell 11:791.
  9. Port, F. & K. Basler (2010) Traffic May 3. [Epub ahead of print].
  10. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
  11. Castelo-Branco, G. et al. (2006) Mol. Cell. Neurosci. 31:251.
  12. Jonsson, M. et al. (1998) Br. J. Cancer 78:430.
  13. van Den Berg, D.J. et al. (1998) Blood 92:3189.
  14. Kruger, C. & C. Kappen (2010) PLoS One 5:e8978.
  15. Lin, G. et al. (2008) Stem Cells Dev. 17:1053.
  16. Lickert, H. et al. (2001) Mech. Dev. 105:181.
  17. Danielson, K.G. et al. (1995) J. Biol. Chem. 270:31225.
  18. Gazit, A. et al. (1999) Oncogene 18:5959.
  19. Bazhin, A. V. et al. (2010) Cell. Mol. Life Sci. 67:817.
  20. Kawasaki, A. et al. (2007) Cell. Signal. 19:2498.
  21. Blumenthal, A. et al. (2006) Blood 108:965.
  22. Umbhauer, M. et al. (2000) EMBO J. 19:4944.
  23. Bryja, V. et al. (2009) Mol. Biol. Cell 20:924.
  24. Keeble, T.R. et al. (2006) J. Neurosci. 26:5840.
  25. Lin, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11196.
  26. Rao, T.P. & M. Kuhl (2010) Circ. Res. 106:1798.
  27. McDonald, S.L. & A. Silver (2009) Br. J. Cancer 101:209.
  28. Cha, S-W. et al. (2009) Curr. Biol. 19:1573.

Publications for Wnt-5a (645-WN)(176)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Sugar Glider, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Control, Differentiation, ELISA Standard, In Vivo, Tissue Culture, Western Blot.


Filter By Application
Binding Assay
(1)
Bioassay
(153)
Cell Culture
(11)
Control
(1)
Differentiation
(4)
ELISA Standard
(1)
In Vivo
(4)
Tissue Culture
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(106)
Mouse
(60)
Rat
(10)
Bovine
(1)
Porcine
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Sugar Glider
(1)
Transgenic Mouse
(1)
Xenopus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 176. Show All 176 Publications.
Publications using 645-WN Applications Species
Hasselluhn, MC;Decker-Farrell, AR;Vlahos, L;Thomas, DH;Curiel-Garcia, A;Maurer, HC;Wasko, UN;Tomassoni, L;Sastra, SA;Palermo, CF;Dalton, TC;Ma, A;Li, F;Tolosa, EJ;Hibshoosh, H;Fernandez-Zapico, ME;Muir, A;Califano, A;Olive, KP; Tumor Explants Elucidate a Cascade of Paracrine SHH, WNT, and VEGF Signals Driving Pancreatic Cancer Angiosuppression Cancer discovery 2023-11-15 [PMID: 37966260] (Bioassay, Mouse) Bioassay Mouse
Yuan, T;Zhang, J;Zhao, Y;Guo, Y;Fan, S; Single-cell RNA sequencing of intestinal crypts reveals vital events in damage repair and the double-edged sword effect of the Wnt3/?-catenin pathway in irradiated mice Redox biology 2023-10-25 [PMID: 37918127] (Bioassay, Mouse) Bioassay Mouse
Riquelme, R;Li, L;Gambrill, A;Barria, A; ROR2 homodimerization is sufficient to activate a neuronal Wnt/calcium signaling pathway The Journal of biological chemistry 2023-10-11 [PMID: 37832874] (Bioassay, Human) Bioassay Human
Tabrizian, N;Nouruzi, S;Cui, CJ;Kobelev, M;Namekawa, T;Lodhia, I;Talal, A;Sivak, O;Ganguli, D;Zoubeidi, A; ASCL1 is activated downstream of the ROR2/CREB signaling pathway to support lineage plasticity in prostate cancer Cell reports 2023-08-07 [PMID: 37552603] (Bioassay, Human) Bioassay Human
Byerly, CD;Patterson, LL;Pittner, NA;Solomon, RN;Patel, JG;Rogan, MR;McBride, JW; Ehrlichia Wnt SLiM ligand mimic deactivates the Hippo pathway to engage the anti-apoptotic Yap-GLUT1-BCL-xL axis Infection and immunity 2023-08-02 [PMID: 37530530] (Bioassay, Human) Bioassay Human
Scalise, M;Marino, F;Salerno, L;Amato, N;Quercia, C;Siracusa, C;Filardo, A;Chiefalo, A;Pagano, L;Misdea, G;Salerno, N;De Angelis, A;Urbanek, K;Viglietto, G;Torella, D;Cianflone, E; Adult Multipotent Cardiac Progenitor-Derived Spheroids: A Reproducible Model of In Vitro Cardiomyocyte Commitment and Specification Cells 2023-07-05 [PMID: 37443827] (Bioassay, Mouse) Bioassay Mouse
Purwaningrum, M;Giachelli, CM;Osathanon, T;Rattanapuchpong, S;Sawangmake, C; Dissecting specific Wnt components governing osteogenic differentiation potential by human periodontal ligament stem cells through interleukin-6 Scientific reports 2023-06-03 [PMID: 37270571] (Bioassay, Human) Bioassay Human
CY Feigin, JA Moreno, R Ramos, SA Mereby, A Alivisatos, W Wang, R van Ameron, J Camacho, JJ Rasweiler, RR Behringer, B Ostrow, MV Plikus, R Mallarino Convergent deployment of ancestral functions during the evolution of mammalian flight membranes Science Advances, 2023-03-24;9(12):eade7511. 2023-03-24 [PMID: 36961889] (Bioassay, Sugar Glider) Bioassay Sugar Glider
CD Byerly, LL Patterson, NA Pittner, RN Solomon, JG Patel, MR Rogan, JW McBride Ehrlichia Wnt short linear motif ligand mimetic deactivates the Hippo pathway to engage the anti-apoptotic Yap-GLUT1-BCL-xL axis bioRxiv : the preprint server for biology, 2023-03-07;0(0):. 2023-03-07 [PMID: 36945589] (Bioassay, Human) Bioassay Human
D Castillo-A, T Wald, EA Reyes, A Gallagher, J Schanin, S Vlachos, N Lamarche-V, C Bomidi, S Blutt, MK Estes, T Nystul, OD Klein A DLG1-ARHGAP31-CDC42 axis is essential for the intestinal stem cell response to fluctuating niche Wnt signaling Cell Stem Cell, 2023-01-13;30(2):188-206.e6. 2023-01-13 [PMID: 36640764] (Bioassay, Mouse) Bioassay Mouse
Show All 176 Publications.

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By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

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Bioinformatics

Gene Symbol WNT5A
Uniprot