Wnt-5a Antibody [DyLight 650]

• Reactivity: Hu, Mu, Rt, Bv, Pm, Am, Gp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 650

Wnt-5a Antibody [DyLight 650] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide corresponding to amino acids 190-230 of human Wnt-5a was used as the immunogen for this antibody, GenBank no NP_003383.2.
• Predicted Species: Guinea Pig (100%), Amphibian (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT5A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-24752. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

The amino acid sequence used as immunogen is 90% homologous in newt (Pleurodeles waltl), and 88% in Xenopus and axolotl (Ambystoma mexicanum). Reactivity to Petarus breviceps reported in verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Wnt-5a Antibody [DyLight 650]

• hWNT5A
• protein Wnt-5a
• wingless-type MMTV integration site family, member 5A
• WNT-5A protein
• Wnt5a
• Wnt-5a

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT5A