Frizzled-2 Antibody

Western Blot: Frizzled-2 Antibody [NBP1-20920] - (0.03ug/ml) staining of Human Heart (A) and (0.1ug/ml) Placenta (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Frizzled-2 Antibody [NBP1-20920] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920] - Staining of paraffin embedded human thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920] - Staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Frizzled-2 Antibody Summary

• Immunogen: Peptide with sequence RYATLEHPFHCPR corresponding to internal region according to NP_001457.1.
• Epitope: internal region
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FZD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 2 - 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03-1 ug/ml
• Application Notes: WB: Preliminary experiments gave an approx. 23 kDa band in human colon, heart and ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6 kDa band according to NP_001457.1. The 23 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Human thyroid gland shows cytoplasm and membranous staining in epithelial cells of the follicles. IHC successful usage on human glioblastomas is reported in scientific literature (PMID: 23748645).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24635767).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Frizzled-2 Antibody

• frizzled (Drosophila) homolog 2
• frizzled homolog 2 (Drosophila)
• Frizzled2
• Frizzled-2
• Fz2
• fz-2
• FZD2
• fzE2
• hFz2

Background

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The expression of the FZD2 gene appears to be developmentally regulated, with high levels of expression in fetal kidney and lung and in adult colon and ovary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Frizzled-2 Antibody (NBP1-20920)(2)

Publications using NBP1-20920

• Kaenel P, Hahnewald S, Wotzkow C et al. Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization. Dev. Growth Differ. 3/18/2014 [PMID: 24635767] (IHC-P, Mouse)
• Oishi I, Kawakami Y, Raya A et al. Regulation of primary cilia formation and left-right patterning in zebrafish by a noncanonical Wnt signaling mediator, duboraya. Nat Genet 2006 Nov [PMID: 17013396]

Bioinformatics

• Gene Symbol: FZD2
• Entrez:
  • 2535(Human)
  • 57265(Mouse)
  • 64512(Rat)
• Uniprot:
  • Q14332(Human)