Frizzled-2 Antibody

Images

 
Western Blot: Frizzled-2 Antibody [NBP1-20920] - (0.03ug/ml) staining of Human Heart (A) and (0.1ug/ml) Placenta (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Frizzled-2 Antibody [NBP1-20920] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920] - Staining of paraffin embedded human thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Frizzled-2 Antibody [NBP1-20920] - Staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Frizzled-2 Antibody Summary

Immunogen
Peptide with sequence RYATLEHPFHCPR corresponding to internal region according to NP_001457.1.
Epitope
internal region
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FZD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 2 - 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03-1 ug/ml
Application Notes
WB: Preliminary experiments gave an approx. 23 kDa band in human colon, heart and ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6 kDa band according to NP_001457.1. The 23 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Human thyroid gland shows cytoplasm and membranous staining in epithelial cells of the follicles. IHC successful usage on human glioblastomas is reported in scientific literature (PMID: 23748645).
Publications
Read Publications using
NBP1-20920 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24635767).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Frizzled-2 Antibody

  • frizzled (Drosophila) homolog 2
  • frizzled homolog 2 (Drosophila)
  • Frizzled2
  • Frizzled-2
  • Fz2
  • fz-2
  • FZD2
  • fzE2
  • hFz2

Background

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The expression of the FZD2 gene appears to be developmentally regulated, with high levels of expression in fetal kidney and lung and in adult colon and ovary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Frizzled-2 Antibody (NBP1-20920)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Mouse
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Frizzled-2 Antibody (NBP1-20920)

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Bioinformatics

Gene Symbol FZD2
Entrez
Uniprot