Wnt-5a: Novus Biologicals

645-WN

Recombinant Human/Mouse Wnt-5a Protein

10 μg

645-WN

645-WN) inhibits Wnt-3a-induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED₅₀ for this effect is 0.100-0.500 µg/mL." class="big_lightbox">

645-WN) inhibits Wnt-3a-induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED₅₀ for this effect is 0.100-0.500 µg/mL." alt="Recombinant mouse Wnt-5a (645-WN) inhibits Wnt-3a-induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED₅₀ for this effect is 0.100-0.500 µg/mL." class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	645-WN-010/CF

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AF645

Wnt-5a Antibody [Unconjugated]

(5 Reviews)

(56 Publications)

100 μg

AF645

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Wnt‑5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Wnt‑5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Mouse/Rat Wnt-5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for Wnt‑5a at approximately ~42kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa Human Cervical Epithelial Carcinoma Cells and Mouse Brain (embryo E14). PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Wnt‑5a at approximately ~42kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

<b>Biological Strategies Validation. </b>NMDAR activation rapidly increases Wnt5a in cortical cultures. A. Cellular localization of Wnt5a in neurons. Shown are confocal images of primary cortical neurons after double-fluorescent immunostaining with anti-Wnt5a (red) and anti-synapsin I (green) antibodies. The nucleus was stained by DAPI (blue). B. MSG or NMDA stimulation increased Wnt5a protein. Primary cortical neurons (10 DIV) were treated with 10 μΜ MSG or 50 μΜ NMDA for 15 min. Wnt5a protein was detected by Western blotting. Data in the summary graphs (mean ± SEM) were from three independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). C. NMDA receptor-regulated Wnt5a increase. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 100 μΜ DAP5 for 30 min, then incubated with 50 μΜ NMDA for 15 min. Wnt5a and p-P70S6K(included as a marker for translation activation) were detected by Western blotting summarized in the graph (n = 3; *, p < 0.05; **, p < 0.01; One-way ANOVA). D. Dynamic expression of Wnt5a protein after NMDA stimulation. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 5, 15, 30 and 60 min followed by Western blotting analysis of Wnt5a (n = 3; *, P < 0.05; **, p < 0.01; One-way ANOVA). E. NMDA-induced Wnt5a protein secretion. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 2, 4, 8, 16 and 32 min, and Wnt5a protein in the media was concentrated and detected on immunoblots. Image collected and cropped by CiteAb from the following open publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>NMDAR activation stimulates Wnt5a protein synthesis via the MAPK signaling pathway. A. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μM PD98059 (PD98) for 30 min, and then stimulated with 50 μΜ NMDA for 15 min. Wnt5a protein was measured by Western blotting and quantified data were presented in graphs (mean ± SEM; n = 3, *p < 0.05; One-way ANOVA). B. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μM U0126 for 30 min, followed by 50 μΜ NMDA for 15 min. Graphs (mean ± SEM) are from three independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). Image collected and cropped by CiteAb from the following open publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of Mov10 increases Wnt5a secretion and Ror2-dependent cell invasion. (a) Wnt5a secretion in conditioned media (CM) from UACC903 melanoma cells compared with whole-cell lysate (WCL) from control and Mov10 shRNA-expressing cells. (b) Wnt5a secretion in UACC903 cells expressing a Wnt5a transgene. (c) Wnt3a secretion in UACC903 cells expressing a Wnt3a transgene. (d) Secretion of fibronectin (FN) from UACC903 cells expressing control and Mov10 shRNA. (e) Collagen invasion of WM239A melanoma cells expressing two Mov10 shRNA constructs. (f) Quantification of invasion assay in (a) Student's t-test, compared with shControl, shMov10-1 P=2.29 × 10−11, shMov10-2 P=3.95 × 10−10. (g) Collagen invasion assay of cells expressing Ror2 shRNA and Mov10 shRNA (see Supplementary Figure S2). (h) Quantification of invasion assay in (d). Student's t-test, shMov10+shCtr vs shCtr P=3.07 × 10−11, shMov10+shRor2-1 vs shMov10+shCtr P=9.02 × 10−10, shMov10+shRor2-2 vs shMov10+shCtr P=1.42 × 10−11. Error bars indicate s.d. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/oncsis201515), licensed under a CC-BY license. Not internally tested by R&D Systems.

PGC depletion in Ror2 and Wnt5a mutants.(A–C) PGCs were visualized by wholemount SSEA1 immunostaining with SSEA1 antibody in e10.25 WT, Ror2, and Wnt5a embryos. (E–G) Gonadal ridges from e11.5 stained with GCNA, and (I–K) e12.5 male gonads stained with GCNA antibody. The caudal end is down in all images. (D, H, L) Quantification of PGCs in the entire e10.25 embryo, e11.5 and e12.5 gonads from confocal stacks, with individuals denoted as WT (diamond), Ror2Y324C mutants (triangle), and Wnt5a null (circle), and means indicated as bars. Consistent with appearances, a significant reduction of PGCs was observed in Wnt5a and from e11.5 onward in Ror2Y324C. We noted no significant difference in the number of PGCs between XX and XY gonads at e12.5 (not shown). Results of the Student's t-test are indicated, * p<0.05, **, p<0.01, ***p<0.001. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pgen.1002428), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>NMDAR-elicited Wnt5a increase requires translation but not transcription. A. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μΜ anisomycin for 30 min, and then incubated with 50 μΜ NMDA for 15 min, followed by Wnt5a immunoblotting. The Graph is a summary of three independent experiments (**, p < 0.01; One-way ANOVA). B. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μΜ actinomycin D for 30 min, followed by addition of 50 μΜ NMDA for 15 min. Wnt5a protein was detected by Western blotting and quantified. The Graph is a summary of four independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). C. Primary cortical neurons (10 DIV) were treated with vehicle (Control) or 50 μΜ NMDA for 15 min. Wnt5a mRNA was quantified by Real-time RT-PCR (qPCR). The summary graph is from three independent experiments (40 cycles, CT values: 25.1 ± 0.5/control vs. 25.6 ± 0.3/NMDA; p > 0.05; two-tailed Student's tests). D. Melt curve of Wnt5a qPCR on control cells. The melt curve on NMDA-stimulated cells was similar (not shown). E. RT-PCR results of Wnt5a in control and NMDA-treated cells. Image collected and cropped by CiteAb from the following open publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>mTOR signaling pathway is not required for the NMDAR-dependent Wnt5a protein synthesis. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 25nΜ Rapamycin for 30 min, followed by addition of 50 μΜ NMDA for 15 min. Western blotting analysis of Wnt5a and phosphor-P70S6K proteins were performed. Graphs (mean ± SEM) are from four independent experiments (*, p < 0.05; **, p < 0.01; #, p > 0.05; One-way ANOVA). Image collected and cropped by CiteAb from the following open publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF645U-100UG
Alexa Fluor 405	AF645V-100UG
Alexa Fluor 488	AF645G-100UG
Alexa Fluor 532	AF645X-100UG
Alexa Fluor 594	AF645T-100UG
Alexa Fluor 647	AF645R-100UG
Alexa Fluor 700	AF645N-100UG
Alexa Fluor 750	AF645S-100UG
Alexa Fluor Plus 405	AF645AFP405-100UG
Alexa Fluor Plus 488	AF645AFP488-100UG
Alexa Fluor Plus 555	AF645AFP555-100UG
Alexa Fluor Plus 594	AF645AFP594-100UG
Alexa Fluor Plus 647	AF645AFP647-100UG
Alexa Fluor Plus 680	AF645AFP680-100UG
Allophycocyanin	AF645A
Biotin	BAF645
CoraFluor 1	AF645CL1
DyLight 350	AF645D
DyLight 405	AF645E
DyLight 488	AF645K
DyLight 550	AF645L
DyLight 594	AF645M
DyLight 650	AF645W
DyLight 680	AF645Y
DyLight 755	AF645Z
FITC	AF645F
HRP	AF645H
Janelia Fluor 525	AF645JF525
Janelia Fluor 549	AF645I
Janelia Fluor 646	AF645J
Janelia Fluor 669	AF645JF669
PE	AF645P
PerCP	AF645C
mFluor Violet 450 SE	AF645MFV450
mFluor Violet 500 SE	AF645MFV500
mFluor Violet 610 SE	AF645MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF645-SP

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MAB645

Wnt-5a Antibody (442625) [Unconjugated]

(1 Review)

(49 Publications)

100 μg

MAB645

NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

WNT-5A expression in mouse GFAP+astrocytes. (A) Immunohistochemistry was performed on adult mouse brain sections using an anti-WNT-5A antibody in combination with anti-glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor protein1 (IBA1) antibodies as astrocyte and microglia marker, respectively. Merge presents the overlay of IBA1, GFAP, WNT-5A. Size bar −2 μm. The images represent a maximum intensity projection of a Z-stack of 5 μm thickness. The white square marked ‘B’ indicates the area magnified in B: (B) Close up of a GFAP+ astrocyte reveals the expression of WNT-5A in this cell type. (C) shows immunoblot detection of recombinant WNT-5A (rWNT-5A; 375 ng/lane) in comparison to lysates from mouse primary microglia and mixed astrocyte cultures. beta -actin serves as a loading control. (D) The bar graph depicts expression levels of WNT-5A mRNA in mouse primary microglia and mixed astrocyte cultures measured by QPCR. Data are normalized to GAPDH expression and analyzed with a non-parametric Mann–Whitney test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 4 to 8. (E) shows indirect immunocytochemistry of mixed astrocyte cultures employing anti-GFAP as astrocyte and anti-CD11b as microglia markers. DAPI is used as nuclear counterstain. Image represents a maximum image projection of an 8 μm Z-stack. Size bar 20 μm. The frame shows 63 cells in total and 10 CD11b-positive microglia (arrows). Routinely 10% to 18% microglia were observed (n = 4). DAPI, 4',6-diamidino-2-phenylindole; GADPH, glyceraldehyde 3-phosphate dehydrogenase; n, number. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22647544), licensed under a CC-BY license. Not internally tested by R&D Systems.

Wnt5a expressing tumors have less Wnt/ beta -catenin signaling than MMTV-Wnt1 tumors.(A) Quantitative RT-PCR of Wnt/ beta -catenin target genes. Expression of Axin2 mRNA in MMTV-Wnt1 versus MMTV-Wnt1;MMTV-Wnt5a tumors as determined by quantitative RT-PCR (n = 5 MMTV-Wnt1, n = 5 MMTV-Wnt1;MMTV-Wnt5a). Data are shown as tables obtained using REST software. Axin2 mRNA was significantly down-regulated in MMTV-Wnt1;MMTV-Wnt5a tumors. (B) Western blot for beta -catenin protein. Protein lysates were prepared from MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a tumors. beta -catenin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as loading controls. The ratio of active beta -catenin to beta -catenin as determined by densitometic analysis is shown. MMTV-Wnt1;MMTV-Wnt5a tumors displayed decreased levels of active beta -catenin compared to controls. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.

Ectopic expression of Wnt5a results in low expression of K6 and K14.Expression of molecular markers for basal and luminal progenitors in MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a mammary glands was compared by western blot. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Each lane contains protein isolated from a separate mouse. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.

Wnt/ beta -catenin signaling is not down-regulated in Wnt5a expressing MMTV-Wnt1 glands.(A) Western blot analysis of beta -catenin protein in MMTV-Wnt1mammary gland. The level of active beta -catenin was compared in protein lysates from MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a mammary glands. Total beta -catenin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as loading controls. The ratio of active beta -catenin-to-beta -catenin is shown at the bottom on the gel. Each lane represents a sample from a different mouse. (B) Quantitative RT-PCR of Axin2, a Wnt/ beta -catenin target gene, in unsorted primary mammary epithelial cells. Expression of Axin2 and hWnt5a mRNA in unsorted PMECs from MMTV-Wnt1;MMTV-Wnt5a vs. MMTV-Wnt1 mammary glands was determined by quantitative RT-PCR (n = 12 MMTV-Wnt1, n = 11 MMTV-Wnt1;MMTV-Wnt5a separate mice). Data are shown as tables obtained using REST analysis software. Expression = fold difference in MMTV-Wnt1;Wnt5a relative to MMTV-Wnt1 controls after normalization to Gapdh. (C) Quantitative RT-PCR of Axin2 in E-cadherin negative primary mammary epithelial cells. Expression of Axin2 and hWnt5a mRNA in E-cadherin (Ecad) negative PMECs from MMTV-Wnt1;MMTV-Wnt5a vs. MMTV-Wnt1 mammary glands was determined by quantitative RT-PCR (n = 2 MMTV-Wnt1, n = 2 MMTV-Wnt1;MMTV-Wnt5a separate mice). Data are shown as tables obtained using REST analysis software. Expression = fold difference in MMTV-Wnt1;Wnt5a relative to MMTV-Wnt1 controls after normalization to Gapdh. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.

Wnt5a expressing tumors demonstrate a decrease in markers of the basal tumor subtype.(A) Western blot using protein lysates isolated from the epithelium of MMTV-Wnt1 and MMTV-1;MMTV-Wnt5a mammary tumors. The expression of molecular markers of basal and luminal tumor subtypes were compared. Keratin 6 and Keratin 5 were strongly down-regulated in Wnt5a expressing tumors. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (B–D) Immunostaining for K6. Sections from MMTV-Wnt1 (B) and MMTV-1;MMTV-Wnt5a (C) tumors were stained with anti-Keratin 6 antibody using immunofluorescence (K6 = green; nuclei = blue). The percentage of cells expressing K6 was determined and graphed (D). Values are means +/− standard error (n = 6 MMTV-Wnt1, 3 fields per tumor; n = 5 MMTV-Wnt1;MMTV-Wnt5a, 3 fields per tumor). MMTV-Wnt1;MMTV-Wnt5a tumors demonstrated a significant decrease in K6-expressing cells as measured by T-test (* = p<0.05). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB645U-100UG
Alexa Fluor 405	FAB645V-100UG
Alexa Fluor 488	FAB645G-100UG
Alexa Fluor 532	FAB645X-100UG
Alexa Fluor 594	FAB645T-100UG
Alexa Fluor 647	FAB645R-100UG
Alexa Fluor 700	FAB645N-100UG
Alexa Fluor 750	FAB645S-100UG
Alexa Fluor Plus 405	FAB645AFP405-100UG
Alexa Fluor Plus 488	FAB645AFP488-100UG
Alexa Fluor Plus 555	FAB645AFP555-100UG
Alexa Fluor Plus 594	FAB645AFP594-100UG
Alexa Fluor Plus 647	FAB645AFP647-100UG
Alexa Fluor Plus 680	FAB645AFP680-100UG
Allophycocyanin	FAB645A
Biotin	FAB645B
CoraFluor 1	FAB645CL1
DyLight 350	FAB645D
DyLight 405	FAB645E
DyLight 488	FAB645K
DyLight 550	FAB645L
DyLight 594	FAB645M
DyLight 650	FAB645W
DyLight 680	FAB645Y
DyLight 755	FAB645Z
FITC	FAB645F
HRP	FAB645H
Janelia Fluor 525	FAB645JF525
Janelia Fluor 549	FAB645I
Janelia Fluor 646	FAB645J
Janelia Fluor 669	FAB645JF669
PE	FAB645P
PerCP	FAB645C
mFluor Violet 450 SE	FAB645MFV450
mFluor Violet 500 SE	FAB645MFV500
mFluor Violet 610 SE	FAB645MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB645-SP

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NBP2-24752

Wnt-5a Antibody - BSA Free

(1 Review)

(16 Publications)

0.1 mg

NBP2-24752

Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP2-24752] - Analysis of Wnt-5a in FFPE human breast tumor using an isotype control (left) and Wnt-5a antibody (right)

Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Wnt-5a antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Reactivity:

Hu, Mu, Rt, Bv, Pm, Am, Gp

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Pm, Am, Gp

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6075A

Alt Catalog# IMG-6075A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24752AF350
Alexa Fluor 405	NBP2-24752AF405
Alexa Fluor 488	NBP2-24752AF488
Alexa Fluor 532	NBP2-24752AF532
Alexa Fluor 594	NBP2-24752AF594
Alexa Fluor 647	NBP2-24752AF647
Alexa Fluor 700	NBP2-24752AF700
Alexa Fluor 750	NBP2-24752AF750
Alexa Fluor Plus 405	NBP2-24752AFP405
Alexa Fluor Plus 488	NBP2-24752AFP488
Alexa Fluor Plus 555	NBP2-24752AFP555
Alexa Fluor Plus 594	NBP2-24752AFP594
Alexa Fluor Plus 647	NBP2-24752AFP647
Alexa Fluor Plus 680	NBP2-24752AFP680
Allophycocyanin	NBP2-24752APC
Biotin	NBP2-24752B
CoraFluor 1	NBP2-24752CL1
DyLight 350	NBP2-24752UV
DyLight 405	NBP2-24752V
DyLight 488	NBP2-24752G
DyLight 550	NBP2-24752R
DyLight 594	NBP2-24752DL594
DyLight 650	NBP2-24752C
DyLight 680	NBP2-24752FR
DyLight 755	NBP2-24752IR
FITC	NBP2-24752F
HRP	NBP2-24752H
Janelia Fluor 525	NBP2-24752JF525
Janelia Fluor 549	NBP2-24752JF549
Janelia Fluor 646	NBP2-24752JF646
Janelia Fluor 669	NBP2-24752JF669
PE	NBP2-24752PE
PerCP	NBP2-24752PCP
mFluor Violet 450 SE	NBP2-24752MFV450
mFluor Violet 500 SE	NBP2-24752MFV500
mFluor Violet 610 SE	NBP2-24752MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24752SS

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NBP1-60032

Wnt-5a Antibody - BSA Free

(1 Publication)

100 ul

NBP1-60032

Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP1-60032] - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP1-60032] - Human Colon, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP1-60032] - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-38893

Human Wnt-5a - Ready-To-Use ELISA Kit (Colorimetric)

1 Kit

NBP3-38893

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.156 - 10 ng/mL (example only; lot dependent)

Sensitivity:

0.072 ng/mL (example only; lot dependent)

Sample Type:

Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids

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H00007474-M04

Wnt-5a Antibody (3A4) - Azide and BSA Free

(4 Publications)

0.1 mg

H00007474-M04

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-24567

Wnt-5a Antibody (CL13683) - BSA Free

100 ul

NBP3-24567

Staining of human salivary gland shows moderate cytoplasmic positivity in glandular cells.

Staining of human cerebellum shows no positivity in Purkinje cells as expected.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43867AF350
Alexa Fluor 405	NBP3-43867AF405
Alexa Fluor 488	NBP3-43867AF488
Alexa Fluor 532	NBP3-43867AF532
Alexa Fluor 594	NBP3-43867AF594
Alexa Fluor 647	NBP3-43867AF647
Alexa Fluor 700	NBP3-43867AF700
Alexa Fluor 750	NBP3-43867AF750
Alexa Fluor Plus 405	NBP3-43867AFP405
Alexa Fluor Plus 488	NBP3-43867AFP488
Alexa Fluor Plus 555	NBP3-43867AFP555
Alexa Fluor Plus 594	NBP3-43867AFP594
Alexa Fluor Plus 647	NBP3-43867AFP647
Alexa Fluor Plus 680	NBP3-43867AFP680
Allophycocyanin	NBP3-43867APC
Biotin	NBP3-43867B
CoraFluor 1	NBP3-43867CL1
DyLight 350	NBP3-43867UV
DyLight 405	NBP3-43867V
DyLight 488	NBP3-43867G
DyLight 550	NBP3-43867R
DyLight 594	NBP3-43867DL594
DyLight 650	NBP3-43867C
DyLight 680	NBP3-43867FR
DyLight 755	NBP3-43867IR
FITC	NBP3-43867F
HRP	NBP3-43867H
Janelia Fluor 525	NBP3-43867JF525
Janelia Fluor 549	NBP3-43867JF549
Janelia Fluor 646	NBP3-43867JF646
Janelia Fluor 669	NBP3-43867JF669
PE	NBP3-43867PE
PerCP	NBP3-43867PCP
mFluor Violet 450 SE	NBP3-43867MFV450
mFluor Violet 500 SE	NBP3-43867MFV500
mFluor Violet 610 SE	NBP3-43867MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-24567-25ul
Azide and BSA Free	NBP3-43867

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NBP3-15684

Wnt-5a Antibody (3P2E8)

100 ul

NBP3-15684

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-38894

Human Wnt-5a ELISA Kit (Colorimetric)

1 Kit

NBP3-38894

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.156 - 10 ng/mL (example only; lot dependent)

Sensitivity:

0.072 ng/mL (example only; lot dependent)

Sample Type:

Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids

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NBP2-24752PEP

Wnt-5a Antibody Blocking Peptide

0.1 mg

Applications:

Applications:

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NBP3-20061

Wnt-5a Antibody (S08-4A3)

100 ul

NBP3-20061

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

WNT5A Products By Gene Name

Recombinant Human/Mouse Wnt-5a Protein

Wnt-5a Antibody [Unconjugated]

Wnt-5a Antibody (442625) [Unconjugated]

Wnt-5a Antibody - BSA Free

Wnt-5a Antibody - BSA Free

Human Wnt-5a - Ready-To-Use ELISA Kit (Colorimetric)

Wnt-5a Antibody (3A4) - Azide and BSA Free

Wnt-5a Antibody (CL13683) - BSA Free

Wnt-5a Antibody (3P2E8)

Human Wnt-5a ELISA Kit (Colorimetric)

Wnt-5a Antibody Blocking Peptide

Wnt-5a Antibody (S08-4A3)