ROR2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ROR2 Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ROR2 at approximately 139 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

ROR2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROR2
Val28-Gly403
Accession # AAA60276
Specificity
Detects human ROR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human ROR1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ROR2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF2064 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROR2 Antibody [Unconjugated]

  • BDB
  • BDB1
  • EC 2.7.10.1
  • MGC163394
  • neurotrophic tyrosine kinase receptor-related 2
  • Neurotrophic tyrosine kinase, receptor-related 2
  • NTRKR2
  • NTRKR2tyrosine-protein kinase transmembrane receptor ROR2
  • receptor tyrosine kinase-like orphan receptor 2
  • ROR2

Background

ROR2 is a type I receptor tyrosine kinase important in chondrocyte, cartilage and growth plate formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROR2 Antibody (AF2064)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Chlorocebus pygerythrus (Vervet Monkey).

We have publications tested in 4 applications: ELISA Development, Flow Cytometry, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(2)
Immunoprecipitation
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF2064 Applications Species
Rasmussen N, Wright T, Brooks S, Hacker K, Debebe Z, Sendor A, Walker M, Major M, Green J, Wahl G, Rathmell W Receptor tyrosine kinase-like orphan receptor 2 (Ror2) expression creates a poised state of Wnt signaling in renal cancer. J Biol Chem, 2013;288(36):26301-10. 2013 [PMID: 23893409] (Western Blot, Human) Western Blot Human
Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex. J. Cell. Sci., 2102;125(0):2017-29. 2102 [PMID: 22328498] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Yang J, Baskar S, Kwong KY Therapeutic Potential and Challenges of Targeting Receptor Tyrosine Kinase ROR1 with Monoclonal Antibodies in B-Cell Malignancies. PLoS ONE, 2011;6(6):e21018. 2011 [PMID: 21698301] (ELISA Development, Human) ELISA Development Human
Feike AC, Rachor K, Gentzel M, Schambony A Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA. Biochem. Biophys. Res. Commun., 2010;400(4):500-6. 2010 [PMID: 20732301] (Immunoprecipitation, Primate - Chlorocebus pygerythrus (Vervet Monkey)) Immunoprecipitation Primate - Chlorocebus pygerythrus (Vervet Monkey)
Safholm A, Tuomela J, Rosenkvist J, Dejmek J, Harkonen P, Andersson T The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility. Clin. Cancer Res., 2008;14(20):6556-63. 2008 [PMID: 18927296] (Western Blot, Human) Western Blot Human
Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia. Clin. Cancer Res., 2008;14(2):396-404. 2008 [PMID: 18223214] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol ROR2
Uniprot