Wnt-3a Antibody (HL1911) - Azide and BSA Free

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (NBP3-25753) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

ICC/IF Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10μm.

Immunohistochemistry-Paraffin Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Rat tissues.Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin Wnt-3a Antibody (HL1911) - Azide and BSA Free [NBP3-25753] - Human prostate cancer.Wnt3a stained by Wnt3a antibody [HL1911] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL1911
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Wnt-3a Antibody (HL1911) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Wnt-3a Antibody (HL1911) - Azide and BSA Free (NBP3-25753) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Wnt-3a.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WNT3A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:1000-1:10000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Wnt-3a Antibody (HL1911) - Azide and BSA Free

• MGC119418
• MGC119419
• MGC119420
• protein Wnt-3a
• wingless-type MMTV integration site family, member 3A
• Wnt3a
• Wnt-3a

Background

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT3A