Wnt-3a Antibodies

1324-WN) induces Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity elicited by Recombinant Mouse Wnt-3a (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB9025). The ND₅₀ is typically 0.15-0.9 µg/mL." class="big_lightbox"> 1324-WN) induces Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity elicited by Recombinant Mouse Wnt-3a (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB9025). The ND₅₀ is typically 0.15-0.9 µg/mL." alt="Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity elicited by Recombinant Mouse Wnt-3a (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB9025). The ND₅₀ is typically 0.15-0.9 µg/mL." class="big_thumb" />

HAF005). A specific band was detected for Wnt-3a at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for Wnt-3a at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with mouse Wnt-3a. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB13242) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-3a at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012)." class="big_lightbox"> MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012)." class="big_thumb" />