Wnt-3a Antibody (217804.2R) [Unconjugated]


Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with mouse Wnt-3a. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Wnt‑3a Monoclonal ...read more
BGO1v human embryonic stem cells were stained with Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB13242, filled histogram) or isotype controlantibody (Catalog # MAB006, open histogram), followed by ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, CyTOF-ready, Flow

Wnt-3a Antibody (217804.2R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
E.coli-derived recombinant mouse Wnt-3a
Ser36-Gln75, Trp219-Arg269
Accession # P27467
Detects mouse Wnt-3a in direct ELISAs.
Details of Functionality
ActivityAssaywCitation not found None None
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/mL

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-3a Antibody (217804.2R) [Unconjugated]

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a


Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).

  1. Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.
  2. Miller, J.R. (2001) Genome Biol. 3:3001.
  3. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  4. Lu, W et al. (2004) Cell 119:97.
  5. Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.
  6. Willert, K. et al. (2003) Nature 423:448.
  7. Komekado, H. et al. (2007) Genes Cells 12:521.
  8. Takada, R. et al. (2006) Dev. Cell 11:791.
  9. Entrez Accession # NP_001100475.
  10. Dunty, W.C. et al. (2008) Development 135:85.
  11. Lee, S.M.K. et al. (2000) Development 127:457.
  12. Takada, S. et al. (1994) Genes Dev. 8:174.
  13. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  14. Nusse, R. (2008) Cell Res. 18:523.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, PLA, RNAi, S-ELISA
Species: Hu
Applications: WB, Flow, CyTOF-ready
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for Wnt-3a Antibody (MAB13242) (0)

There are no publications for Wnt-3a Antibody (MAB13242).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Wnt-3a Antibody (MAB13242) (0)

There are no reviews for Wnt-3a Antibody (MAB13242). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Wnt-3a Antibody (MAB13242) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 IC13242U
Alexa Fluor 405 IC13242V
Alexa Fluor 488 IC13242G
Alexa Fluor 594 IC13242T
Alexa Fluor 647 IC13242R
Alexa Fluor 700 IC13242N
Alexa Fluor 750 IC13242S

Additional Wnt-3a Products

Diseases for Wnt-3a Antibody (MAB13242)

Discover more about diseases related to Wnt-3a Antibody (MAB13242).

Pathways for Wnt-3a Antibody (MAB13242)

View related products by pathway.

PTMs for Wnt-3a Antibody (MAB13242)

Learn more about PTMs related to Wnt-3a Antibody (MAB13242).

Blogs on Wnt-3a

There are no specific blogs for Wnt-3a, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Wnt-3a Antibody (217804.2R) [Unconjugated] and receive a gift card or discount.