Wnt-3a Antibody (217804.2R) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
217804.2R
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 405

Wnt-3a Antibody (217804.2R) [Alexa Fluor® 405] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E.coli-derived recombinant mouse Wnt-3a
Ser36-Gln75, Trp219-Arg269
Accession # P27467
Specificity
Detects mouse Wnt-3a in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-3a Antibody (217804.2R) [Alexa Fluor® 405]

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Background

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).

  1. Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.
  2. Miller, J.R. (2001) Genome Biol. 3:3001.
  3. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  4. Lu, W et al. (2004) Cell 119:97.
  5. Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.
  6. Willert, K. et al. (2003) Nature 423:448.
  7. Komekado, H. et al. (2007) Genes Cells 12:521.
  8. Takada, R. et al. (2006) Dev. Cell 11:791.
  9. Entrez Accession # NP_001100475.
  10. Dunty, W.C. et al. (2008) Development 135:85.
  11. Lee, S.M.K. et al. (2000) Development 127:457.
  12. Takada, S. et al. (1994) Genes Dev. 8:174.
  13. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  14. Nusse, R. (2008) Cell Res. 18:523.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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