Wnt-1 Antibody

Product Discontinued

Wnt-1 Antibody Summary

• Immunogen: Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).
• Localization: Possibly secreted and associates with the extracellular matrix.
• Specificity: Wnt1
• Isotype: IgG1
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: IHC-P: Use at a dilution of 1/100 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.WB: Use at an assay dependent dilution. Predicted molecular weight: 41 kDa.
• Control:

  NIH 3T3 Whole Cell Lysate
  MCF-7 Whole Cell Lysate

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4°C.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Wnt-1 Antibody

• Int-1
• INT1Proto-oncogene Int-1 homolog
• proto-oncogene Wnt-1
• wingless-type MMTV integration site family, member 1 (oncogene INT1)
• wingless-type MMTV integration site family, member 1
• Wnt1
• Wnt-1

Background

Wnt1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta catenine. Mature human Wnt1 is a glycosylated protein containing 343 amino acid residues. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT1
• Entrez:
  • 7471(Human)