WDR5 Antibody

Western Blot: WDR5 Antibody [NBP1-30070] - Detection of mouse and rat WDR5 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody NBP1-30070 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30070] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- WDR5 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: WDR5 Antibody [NBP1-30070] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. WDR5 was successfully IPed by NBP1-30063.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, Simple Western, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

WDR5 Antibody Summary

• Description: Novus Biologicals Rabbit WDR5 Antibody (NBP1-30070) is a polyclonal antibody validated for use in IHC, WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: maps to a region between residue 50 and 100 of human WD repeat domain 5 using the numbering given in entry NP_060058.1
• Predicted Species: Bovine (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WDR5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Simple Western
  • Western Blot 1:2000-1:10000
• Application Notes: IP Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  See Simple Western Antibody Database for Simple Western validation: tested in Human Cancer Cell Line; antibody dilution of 1:10; separated by size; detects a band at 47 kDa

Reactivity Notes

D. melanogaster, X. tropicalis (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WDR5 Antibody

• BIG3
• BIG-3
• BMP2-induced 3-kb gene protein
• SWD3
• SWD3, Set1c WD40 repeat protein, homolog
• WD repeat domain 5
• WD repeat-containing protein 5
• WDR5
• WD-repeat protein 5

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WDR5