RbBP5 Antibody

Images

 
Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug for WB and Input; one 10 cm plate for IP) from 293T cells. Antibody used at the indicated concentrations for WB or 10 ug for IP (15% of IP loaded). ...read more
Immunohistochemistry: RbBP5 Antibody [NB600-252] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - ChIP-chip scatter plot of anti-RbBP5 enriched DNA binding sites versus input reference DNA. A. 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RbBP5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 538) of human retinoblastoma binding protein 5 using the numbering given in entry NP_005048.2 (GeneID 5929).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBBP5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:20000
  • Chromatin Immunoprecipitation 10ug
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Reported use in ChIP assays (see Dou et al). Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NB600-252 in the following applications:

Publications
Read Publications using
NB600-252 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RbBP5 Antibody

  • RBQ3RBBP-5
  • retinoblastoma binding protein 5
  • Retinoblastoma-binding protein RBQ-3
  • Set1c WD40 repeat protein, homolog
  • SWD1

Background

The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RbBP5 Antibody (NB600-252)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(2)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB600-252 Applications Species
Bieluszewska A, Weglewska M, Bieluszewski T et al. PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein FEBS J. 2017 Dec 30 [PMID: 29288530] (WB, Human) WB Human
Dhawan S, Dirice E, Kulkarni RN, Bhushan A. Inhibition of TGF-beta signaling promotes human pancreatic beta cell replication. Diabetes. 2016 Mar 02 [PMID: 26936960] (WB, Human) WB Human
Ma P, Pan H, Montgomery RL et al. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A 2012 Feb [PMID: 22223663]
Ram O, Goren A, Amit I et al. Combinatorial patterning of chromatin regulators uncovered by genome-wide location analysis in human cells. Cell 2011 Dec [PMID: 22196736]
Lin JJ, Lehmann LW, Bonora G et al. Mediator coordinates PIC assembly with recruitment of CHD1. Genes Dev 2011 Oct [PMID: 21979373]
Li X, Wang S, Li Y et al. Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes. Blood 2011 Aug [PMID: 21653943]
Wang GG, Song J, Wang Z et al. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Nature 2009 Jun [PMID: 19430464]
Wen H, Dou Y, Hogaboam CM et al. Epigenetic regulation of dendritic cell-derived interleukin-12 facilitates immunosuppression after a severe innate immune response. Blood 2008 Feb [PMID: 18055863]
Lan F, Bayliss PE, Rinn JL et al. A histone H3 lysine 27 demethylase regulates animal posterior development. Nature 2007 Oct [PMID: 17851529]
De Santa F, Totaro MG, Prosperini E et al. The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing. Cell 2007 Sep [PMID: 17825402]
Show All 13 Publications.

Review for RbBP5 Antibody (NB600-252) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-252:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Koustav Sarkar
WB Human 03/26/2015
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

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WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Koustav Sarkar
03/26/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol RBBP5
Entrez
Uniprot