VPS37B Antibody - BSA Free

Western Blot: VPS37B Antibody [NBP3-06284] - Anti-VPS37B rabbit polyclonal antibody at 1:500 dilutionLane A: RT4 Whole Cell LysateLane B: U251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284] - Immunochemical staining of human VPS37B in human rectum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: VPS37B Antibody [NBP3-06284] - Immunochemical staining of human VPS37B in human gallbladder with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: VPS37B Antibody [NBP3-06284] - VPS37B was immunoprecipitated using:Lane A:0.5 mg U251 MG Whole Cell Lysate4 uL anti-VPS37B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-VPS37B rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :35 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VPS37B Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human VPS37B fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS37B
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for VPS37B Antibody - BSA Free

• ESCRT-I complex subunit VPS37B
• FLJ12750
• hVps37B
• vacuolar protein sorting 37 homolog B (S. cerevisiae)
• vacuolar protein sorting 37B (yeast)
• vacuolar protein sorting 37B
• vacuolar protein sorting-associated protein 37B

Background

VPS37B is a component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting ofendocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS37B