VPS37C Antibody

Images

 
Western Blot: VPS37C Antibody [NB100-2438] - (0.03g/ml) staining of human duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: VPS37C Antibody [NB100-2438] - (3.8ug/ml) staining of Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VPS37C Antibody Summary

Immunogen
Peptide with sequence C-ETLKDKTLQELEELQ corresponding to internal region according to NP_060436.4.
Epitope
C-ETLKDKTLQELEELQ
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VPS37C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 1:200
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 38 kDa band observed in human duodenum lysates (calculated MW of 38.7 kDa band according to NP_060436.4). IHC-P: Human prostate shows strong cytoplasm staining in stromal cells.
Positive Control
Duodenum Lysate (NB820-59212)
Publications
Read Publication using NB100-2438.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS37C Antibody

  • ESCRT-I complex subunit VPS37C
  • FLJ20847
  • hVps37C
  • PML39
  • vacuolar protein sorting 37 homolog C (S. cerevisiae)
  • vacuolar protein sorting 37C (yeast)
  • vacuolar protein sorting 37C
  • vacuolar protein sorting-associated protein 37C

Background

VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS37C Antibody (NB100-2438)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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VPS37C NB100-2438

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Bioinformatics

Gene Symbol VPS37C
Entrez
Uniprot