VPS33A Antibody

Images

 
Western Blot: VPS33A Antibody [NBP2-20872] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS33A antibody [C1C3] (119416) diluted at 1:500. The signal was ...read more
Immunocytochemistry/ Immunofluorescence: VPS33A Antibody [NBP2-20872] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS33A protein stained by VPS33A antibody [C1C3] diluted at 1:200. Red: alpha ...read more
Immunohistochemistry-Paraffin: VPS33A Antibody [NBP2-20872] - Immunohistochemical analysis of paraffin-embedded colon ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

VPS33A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human VPS33A. The exact sequence is proprietary.
Localization
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Lysosome membrane; Peripheral membrane protein; Cytoplasmic side (By similarity)
Predicted Species
Rat (96%), Porcine (97%), Bovine (97%), Rhesus Macaque (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS33A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Zebrafish (83%), Xenopus laevis (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS33A Antibody

  • FLJ22395
  • FLJ23187
  • hVPS33A
  • vacuolar protein sorting 33 homolog A (S. cerevisiae)
  • vacuolar protein sorting 33A (yeast)
  • vacuolar protein sorting 33A
  • vacuolar protein sorting-associated protein 33A

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for VPS33A Antibody (NBP2-20872)

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Bioinformatics

Gene Symbol VPS33A
Uniprot