VPS41 Antibody

Images

 
Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Prostate. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Kidney. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were ...read more
Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Placenta. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VPS41 Antibody Summary

Immunogen
Peptide with sequence C-DHLKKDSQNKTLLK corresponding to internal region according to NP_055211.2, NP_542198.2.
Specificity
This antibody is expected to recognise both reported isoforms (NP_055211.2 and NP_542198.2)
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VPS41
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 - 6 ug/mL
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave bands at approx. 48 kDa band and 28 kDa band in human duodenum, kidney and placenta lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5 kDa band according to NP_055211.1 and 100 kDa band according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human kidney shows staining of collective tubules.
Theoretical MW
99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
VPS41 Overexpression Lysate
Publications
Read Publications using
NB100-2425 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS41 Antibody

  • HVPS41
  • hVps41p
  • HVSP41
  • S53
  • vacuolar assembly protein 41
  • vacuolar protein sorting 41 (yeast homolog)
  • vacuolar protein sorting 41 (yeast)
  • vacuolar protein sorting 41 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 41 homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS41 Antibody (NB100-2425)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for VPS41 Antibody (NB100-2425)

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Blogs on VPS41.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

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Bioinformatics

Gene Symbol VPS41
Entrez
Uniprot