VPS54 Antibody - Azide and BSA Free

Western Blot: VPS54 Antibody [H00051542-B01P] - Analysis of VPS54 expression in transfected 293T cell line by VPS54 polyclonal antibody. Lane 1: VPS54 transfected lysate(90.64 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VPS54 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse VPS54 Antibody - Azide and BSA Free (H00051542-B01P) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-VPS54 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: VPS54 (AAH41868.1, 1 a.a. - 824 a.a.) full-length human protein. MLPTKNRIKREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSDLNRPLEDDCQVLEECVINKVSQTEEIDTDVVVKLADQMRMLNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKDNSLDTEVAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPCSSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSELGKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQICGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSLSDGKIALPEKKSGATEERKPAEVLIVEGQQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQYSMLRHFGHITKDYHDHIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSACFRNICKQMTKMHEAIFDHLPEEQTQMLFLRINASYKLHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR
• Specificity: Reacts with vacuolar protein sorting 54 homolog (S. cerevisiae).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: VPS54
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in western blot.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VPS54 Antibody - Azide and BSA Free

• HCC8SLP-8p
• Hepatocellular carcinoma protein 8
• hVps54L
• Tumor antigen HOM-HCC-8
• Tumor antigen SLP-8p
• vacuolar protein sorting 54 (yeast)
• vacuolar protein sorting 54 homolog (S. cerevisiae)
• vacuolar protein sorting-associated protein 54
• VPS54L

Background

This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS54 Antibody (H00051542-B01P)(1)

Publication using H00051542-B01P

• Berruti G, Ripolone M, Ceriani M. USP8, a regulator of endosomal sorting, is involved in mouse acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Biol Reprod 82(5):930-9. 2010-05-01 [PMID: 20130268]

Bioinformatics

• Gene Symbol: VPS54
• Entrez:
  • 51542(Human)
• Uniprot:
  • AAH41868.1(Human)