VPS35 Antibody

Images

 
Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Western Blot: VPS35 Antibody [NB100-1397] - 10 ug cell lysate per lane of various cell lines as indicated, immunoblotted with vps35 antibody at 1:2000 at 4C for 20 hrs. Secondary antibody donkey anti-goat HRP at 1:5000 ...read more
Western Blot: VPS35 Antibody [NB100-1397] - HEK293T cell lysate. WB image submitted by a verified customer review.
Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human (A) Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397] - Confocal microscopy image of human RPE-1 cells fixed with PFA, permeablilized with 0.3% Triton X-100,then immunostained with vps35 antibody at ...read more
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, ChHa, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VPS35 Antibody Summary

Immunogen
Peptide with sequence C-SPESEGPIYEGLIL corresponding to C-Terminus according to NP_060676.2.
Specificity
Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VPS35
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 8 ug/mL
  • Immunohistochemistry-Paraffin 8 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.03 - 0.1 ug/mL
Application Notes
Western blot: Approx 90 kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 91.7 kDa according to NP_060676.2).
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
VPS35 Overexpression Lysate
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB100-1397 in the following applications:

Publications
Read Publications using
NB100-1397 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine. It has been reported in the literature to react with Chinese hamster ovary cells (PMID: 22747682). Mouse reactivity reported in scientific literature (PMID: 28252385). Canine, chicken and rat reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS35 Antibody

  • DKFZp434P1672
  • FLJ10752
  • FLJ13588
  • FLJ20388
  • hVPS35
  • Maternal-embryonic 3
  • MEM3DKFZp434E1211
  • vacuolar protein sorting 35 (yeast homolog)
  • vacuolar protein sorting 35 (yeast)
  • vacuolar protein sorting 35 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 35
  • Vesicle protein sorting 35

Background

Chemokine receptor D6 expression has been documented in human skin, small intestine, large intestine, appendix, lymph node, vessels, tonsil, spleen, as well as from rat brain tissues. ESTs have been isolated from cancerous human lung, uterus, cervix, placenta, blood, and from normal human placenta libraries. Caution: G protein-coupled receptor D6 was previously called CCR10 and/or CCR9. CCR9 and C-C chemokine receptor 9 referred to both chemokine receptor D6 and the unrelated receptor GPR-9-6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS35 Antibody (NB100-1397)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Human;Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(9)
IHC
(1)
IHC-P
(1)
WB
(11)
All Applications
Filter By Species
Human
(8)
Mouse
(5)
Human;Mouse
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB100-1397 Applications Species
Cui Y, Yang Z, Flores-Rodriguez N et al. Formation of retromer transport carriers is disrupted by the Parkinson disease-linked Vps35 D620N variant Traffic (Copenhagen, Denmark) Dec 21 2020 [PMID: 33347683]
Han J, Goldstein LA, Hou W et al. Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport Autophagy May 25 2020 [PMID: 32397873] (WB, Mouse) WB Mouse
Yasa S, Modica G, Sauvageau E Et Al. CLN3 regulates endosomal function by modulating Rab7A effector interactions J. Cell. Sci. Feb 7 2020 [PMID: 32034082] (WB, Human) WB Human
Tammineni P, Jeong Y Y et al. Impaired axonal retrograde trafficking of the retromer complex augments lysosomal deficits in Alzheimer's disease neurons. Hum Mol Genet Nov 15 2017 [PMID: 28973312] (IHC, WB, Mouse) IHC, WB Mouse
Wu S, Fagan RR, Uttamapinant C et al. The Dopamine Transporter Recycles via a Retromer-Dependent Postendocytic Mechanism: Tracking Studies Using a Novel Fluorophore-Coupling Approach J Neurosci. 2017 Sep 26 [PMID: 28847807] (IHC-P, Mouse) IHC-P Mouse
Lobingier BT, Huttenhain R, Eichel K et al. An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Cell. Apr 6 2017 [PMID: 28388416]
Elwell CA, Czudnochowski N, von Dollen J et al. Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction. Elife. 2017 Mar 02 [PMID: 28252385] (WB, Mouse) WB Mouse
Paul B, Kim HS, Kerr MC et al. Structural basis for the hijacking of endosomal sorting nexin proteins by Chlamydia trachomatis. Elife. 2017 Feb 22 [PMID: 28226239] (Human) Human
Yin J, Liu X, He Q et al. Vps35-dependent recycling of Trem2 regulates microglial function. Traffic Sep 26 2016 [PMID: 27717139]
Yang Z, Hong LK, Follett J et al. Functional characterization of retromer in GLUT4 storage vesicle formation and adipocyte differentiation. FASEB J. 2015 Nov 18 [PMID: 26581601] (WB, ICC/IF, Human;Mouse) WB, ICC/IF Human;Mouse
Show All 23 Publications.

Reviews for VPS35 Antibody (NB100-1397) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Rat, Canine, Chicken, Human.

Reviews using NB100-1397:
Filter by Applications
IF
(1)
Immunocytochemistry
(2)
Western Blot
(2)
All Applications
Filter by Species
Human
(3)
Rat
(1)
Canine, Chicken, Human
(1)
All Species
Images Ratings Applications Species Date Details
IF VPS35 NB100-1397
Enlarge
5
reviewed by:
Verified Customer
IF Rat 10/29/2020
View

Summary

ApplicationIF
Sample TestedINS-1
SpeciesRat
LotC3 E021219
Immunocytochemistry VPS35 NB100-1397
Enlarge
4
reviewed by:
Verified Customer
Immunocytochemistry Human 05/28/2020
View

Summary

ApplicationImmunocytochemistry
Sample Testedhela cell
SpeciesHuman
LotC3 E021219
Western Blot VPS35 NB100-1397
Enlarge
5
reviewed by:
jerome cattin
Western Blot Human 05/28/2020
View

Summary

ApplicationWestern Blot
Sample TestedHEK293T cell lysate
SpeciesHuman
LotC3 E0212129
Western Blot VPS35 NB100-1397
Enlarge
5
reviewed by:
Stephen Terry
Western Blot Canine, Chicken, Human 01/07/2020
View

Summary

ApplicationWestern Blot
Sample TestedDF-1,MDCK canine kidney epithelial cell line,RPE-1,SH-SY5Y whole cell lysates
SpeciesCanine, Chicken, Human
LotC3E230818

Comments

Commentssamples prepared in 1.5x lammeli buffer, 10ug of protein loaded per lane
transferred onto nitrocellulose at 100V for 1.5 hours 4 degrees.

blotting coditions
membrane blocked in 5% milk in PBS 0.1% tween 20 (PBST) for 1 hour
primary ab incubated 1:2000 in 5% milk in PBS at 4 degrees for 20 hours
washed 3X with PBST
secondary donkey anti goat HPR 1:5000 for 1 hour room temp washed 2x PBST, 1X PBS
blot developed using ECL prime reagent (GE healthcare )
Immunocytochemistry VPS35 NB100-1397
Enlarge
5
reviewed by:
Stephen Terry
Immunocytochemistry Human 01/07/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedRPE-1 cells,RPE-1
SpeciesHuman
LotC3E230818

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VPS35 Antibody (NB100-1397) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Verified Customer
10/29/2020
Application: IF
Species: Rat

Verified Customer
05/28/2020
Application: Immunocytochemistry
Species: Human

jerome cattin
05/28/2020
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol VPS35
Entrez
Uniprot