VPS35 Antibody


Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human Cerebellum (A) and Mouse Brain (B) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more

Product Details

Reactivity Hu, Mu, Rt, ChHaSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
0.5 mg/ml

Order Details

VPS35 Antibody Summary

Peptide with sequence C-SPESEGPIYEGLIL corresponding to C-Terminus according to NP_060676.2.
Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Immunogen affinity purified
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  • Western Blot 1 - 3 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
Application Notes
IHC-P has been validated for some of the previous lots.
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
VPS35 Lysate (NBL1-17746)
Brain Lysate (NB820-59177)
Read Publications using
NB100-1397 in the following applications:

  • 8 publications
  • WB
    7 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine. It has been reported in the literature to react with Chinese hamster ovary cells. PMID: 22747682. Mouse reactivity reported in scientific literature (PMID: 28252385).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3), 0.5% BSA
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified

Alternate Names for VPS35 Antibody

  • DKFZp434P1672
  • FLJ10752
  • FLJ13588
  • FLJ20388
  • hVPS35
  • Maternal-embryonic 3
  • MEM3DKFZp434E1211
  • vacuolar protein sorting 35 (yeast homolog)
  • vacuolar protein sorting 35 (yeast)
  • vacuolar protein sorting 35 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 35
  • Vesicle protein sorting 35


Chemokine receptor D6 expression has been documented in human skin, small intestine, large intestine, appendix, lymph node, vessels, tonsil, spleen, as well as from rat brain tissues. ESTs have been isolated from cancerous human lung, uterus, cervix, placenta, blood, and from normal human placenta libraries. Caution: G protein-coupled receptor D6 was previously called CCR10 and/or CCR9. CCR9 and C-C chemokine receptor 9 referred to both chemokine receptor D6 and the unrelated receptor GPR-9-6.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P
Species: Hu
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Publications for VPS35 Antibody (NB100-1397)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Human;Mouse.

We have publications tested in 2 applications: ICC/IF, WB.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-1397 Applications Species
Wu S, Fagan RR, Uttamapinant C et al. The Dopamine Transporter Recycles via a Retromer-Dependent Postendocytic Mechanism: Tracking Studies Using a Novel Fluorophore-Coupling Approach. J. Neurosci. Sep 27 2017 [PMID: 28847807] (ICC/IF, Mouse) ICC/IF Mouse
Wu S, Fagan RR, Uttamapinant C et al. The Dopamine Transporter Recycles Via A Retromer-Dependent Post-Endocytic Mechanism: Tracking Studies Using A Novel Fluorophore-Coupling Approach Journal of Neuroscience Sep 27 2017 [PMID: 28847807] (ICC/IF, Mouse) ICC/IF Mouse
Lobingier BT, Huttenhain R, Eichel K et al. An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Cell. Apr 6 2017 [PMID: 28388416]
Elwell CA, Czudnochowski N, von Dollen J et al. Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction. Elife. 2017 Mar 02 [PMID: 28252385] (WB, Mouse) WB Mouse
Paul B, Kim HS, Kerr MC et al. Structural basis for the hijacking of endosomal sorting nexin proteins by Chlamydia trachomatis. Elife. 2017 Feb 22 [PMID: 28226239] (Human) Human
Yin J, Liu X, He Q et al. Vps35-dependent recycling of Trem2 regulates microglial function. Traffic Sep 26 2016 [PMID: 27717139]
Yang Z, Hong LK, Follett J et al. Functional characterization of retromer in GLUT4 storage vesicle formation and adipocyte differentiation. FASEB J. 2015 Nov 18 [PMID: 26581601] (WB, ICC/IF, Human;Mouse) WB, ICC/IF Human;Mouse
Yin P, Hong Z, Yang X et al. A role for retromer in hepatitis C virus replication. Cell. Mol. Life Sci. 2015 Aug 23 [PMID: 26298293] (ICC/IF, Human)

VPS35 antibody (Imgenex: IMG-3575) was used for ICC-IF analysis of Huh 7.5.1 cells, JFH1-plasmid infected Huh 7.5.1 cells, OR6 cells (which harbors the full-length genotype 1b HCV RNA) and Cured OR6 cells (OR6 cells treated with 10 IU/ml of IFN alpha for 7 days) [Fig 2A/B, 3A/B].
ICC/IF Human
Hsiao JC, Chu LW, Lo YT et al. Intracellular Transport of Vaccinia Virus in HeLa Cells Requires WASH-VPEF/FAM21-Retromer Complexes and Recycling molecules Rab11 and Rab22 J. Virol. 2015 Jun 03 [PMID: 26041286] (WB, ICC/IF, Human)

VPS35 antibody was used for ICC-IF on HeLa cells that were transfected with a plasmid expressing mock/GFP or GFP-VPS26. The antibody was also used for WB application on lysates of HeLa cells which were either mock transfected /Control or transfected with siRNA duplexes targeting CypB (Si-CypB), SNX1 (Si-SNX1), WASH (Si-WASH), or Vps35 (Si-Vps35) [FIG 6].
WB, ICC/IF Human
Fuse A, Furuya N, Kakuta S et al. VPS29-VPS35 intermediate of retromer is stable and may be involved in the retromer complex assembly process. FEBS Lett. 2015 Jun 04 [PMID: 25937119] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 16 Publications.

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Gene Symbol VPS35