Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunocytochemistry: VPS35 Antibody [NB100-1397] - Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University ...read more
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry: VPS35 Antibody [NB100-1397] - Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB:Approx 90kDa band observed in Human Brain (cerebellum), Heart and Rat Brain lysates (calculated MW of 91.7kDa according to NP_060676.2). IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis. Data obtained with a previous batch. Immunocytochemistry: Vesicular staining of VPS35 (green) in HEK293 and in SHSY5Y. Based on these data, this antibody was endorsed by PabmAbs (http://pabmabs.com/wordpress/?p=1523).
91 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted cross-reactivity based on sequence identity: Bovine. It has been reported in the literature to react with Chinese hamster ovary cells. PMID: 22747682. Mouse reactivity reported in scientific literature (PMID: 28252385).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for VPS35 Antibody
vacuolar protein sorting 35 (yeast homolog)
vacuolar protein sorting 35 (yeast)
vacuolar protein sorting 35 homolog (S. cerevisiae)
vacuolar protein sorting-associated protein 35
Vesicle protein sorting 35
Chemokine receptor D6 expression has been documented in human skin, small intestine, large intestine, appendix, lymph node, vessels, tonsil, spleen, as well as from rat brain tissues. ESTs have been isolated from cancerous human lung, uterus, cervix, placenta, blood, and from normal human placenta libraries. Caution: G protein-coupled receptor D6 was previously called CCR10 and/or CCR9. CCR9 and C-C chemokine receptor 9 referred to both chemokine receptor D6 and the unrelated receptor GPR-9-6.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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