Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Western Blot: VPS35 Antibody [NB100-1397] - 10 ug cell lysate per lane of various cell lines as indicated, immunoblotted with vps35 antibody at 1:2000 at 4C for 20 hrs. Secondary antibody donkey anti-goat HRP at 1:5000 ...read more
Western Blot: VPS35 Antibody [NB100-1397] - HEK293T cell lysate. WB image submitted by a verified customer review.
Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human (A) Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397] - Confocal microscopy image of human RPE-1 cells fixed with PFA, permeablilized with 0.3% Triton X-100,then immunostained with vps35 antibody at ...read more
Immunocytochemistry/ Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Western blot: Approx 90 kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 91.7 kDa according to NP_060676.2).
91 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted cross-reactivity based on sequence identity: Bovine. It has been reported in the literature to react with Chinese hamster ovary cells (PMID: 22747682). Mouse reactivity reported in scientific literature (PMID: 28252385). Canine, chicken and rat reactivity reported from verified customer reviews.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for VPS35 Antibody
vacuolar protein sorting 35 (yeast homolog)
vacuolar protein sorting 35 (yeast)
vacuolar protein sorting 35 homolog (S. cerevisiae)
vacuolar protein sorting-associated protein 35
Vesicle protein sorting 35
Chemokine receptor D6 expression has been documented in human skin, small intestine, large intestine, appendix, lymph node, vessels, tonsil, spleen, as well as from rat brain tissues. ESTs have been isolated from cancerous human lung, uterus, cervix, placenta, blood, and from normal human placenta libraries. Caution: G protein-coupled receptor D6 was previously called CCR10 and/or CCR9. CCR9 and C-C chemokine receptor 9 referred to both chemokine receptor D6 and the unrelated receptor GPR-9-6.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
samples prepared in 1.5x lammeli buffer, 10ug of protein loaded per lane transferred onto nitrocellulose at 100V for 1.5 hours 4 degrees.
blotting coditions membrane blocked in 5% milk in PBS 0.1% tween 20 (PBST) for 1 hour primary ab incubated 1:2000 in 5% milk in PBS at 4 degrees for 20 hours washed 3X with PBST secondary donkey anti goat HPR 1:5000 for 1 hour room temp washed 2x PBST, 1X PBS blot developed using ECL prime reagent (GE healthcare )
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