| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IHC-P |
| Clone | F8/86 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 680 |
| Immunogen | von Willebrand Factor isolated from human plasma |
| Localization | Cytoplasmic |
| Marker | Endothelial Marker |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | VWF |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![Von Willebrand Factor Antibody (F8/86) [DyLight 680]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Von Willebrand Factor Antibody (F8/86) [DyLight 680]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54379AF350 | |
| Alexa Fluor 405 | NBP2-54379AF405 | |
| Alexa Fluor 488 | NBP2-54379AF488 | |
| Alexa Fluor 532 | NBP2-54379AF532 | |
| Alexa Fluor 594 | NBP2-54379AF594 | |
| Alexa Fluor 647 | NBP2-54379AF647 | |
| Alexa Fluor 700 | NBP2-54379AF700 | |
| Alexa Fluor 750 | NBP2-54379AF750 | |
| Allophycocyanin | NBP2-54379APC | |
| Biotin | NBP2-54379B | |
| DyLight 350 | NBP2-54379UV | |
| DyLight 405 | NBP2-54379V | |
| DyLight 488 | NBP2-54379G | |
| DyLight 550 | NBP2-54379R | |
| DyLight 594 | NBP2-54379DL594 | |
| DyLight 650 | NBP2-54379C | |
| DyLight 680 | NBP2-54379FR | |
| DyLight 755 | NBP2-54379IR | |
| FITC | NBP2-54379F | |
| HRP | NBP2-54379H | |
| Janelia Fluor 549 | NBP2-54379JF549 | |
| Janelia Fluor 646 | NBP2-54379JF646 | |
| PE | NBP2-54379PE | |
| PerCP | NBP2-54379PCP |
Diseases for vWF-A2 Antibody (NBP2-54379FR)Discover more about diseases related to vWF-A2 Antibody (NBP2-54379FR).
| Pathways for vWF-A2 Antibody (NBP2-54379FR)View related products by pathway.
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PTMs for vWF-A2 Antibody (NBP2-54379FR)Learn more about PTMs related to vWF-A2 Antibody (NBP2-54379FR).
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Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the... Read full blog post. |
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Von Willebrand Factor: An important mediator and carrier of hemostasis Human Von Willebrand Factor (factor VIII R: Ag) is a 270 kDa multimeric plasma gylcoprotein. Important in the maintenance of hemostasis, it mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation facto... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | VWF |
![Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).](http://images.novusbio.com/images2/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/images2/Factor-VIII-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91761-img0005.jpg)
![Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.](http://images.novusbio.com/images2/Factor-VIII-Antibody-Immunohistochemistry-Paraffin-NB100-91761-img0007.jpg)
![Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Immunoblots showing protein expression of ALK5, pSMAD2/3, PAI-1, pVHL, andI2-actin in A498 cells after treatment with TGF-i2 at given point of time. Image collected and cropped by Cite Ab from the following publication (VHL status regulates transforming growth factor-β signaling pathways in renal cell carcinoma. Oncotarget (2018)) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Serpin-E1-PAI-1-Antibody-Western-Blot-NBP1-19773-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP1-19773] - PAI1/Serpine1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/images2/Serpin-E1-PAI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19773-img0006.jpg)
![Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP1-19773] - Staining of PAI1/Serpine 1 in mouse pancreas.](http://images.novusbio.com/images2/Serpin-E1-PAI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-19773-img0007.jpg)
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/images2/Tenascin-C-Antibody-(4C8MS)-Flow-(Intracellular)-NB110-68136-img0010.jpg)
![Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Tenascin-C-Antibody-(4C8MS)-Western-Blot-NB110-68136-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Tenascin-C-Antibody-(4C8MS)-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Western-Blot-NBP1-58268-img0007.jpg)
![Immunohistochemistry: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Analysis of human liver after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-NBP1-58268-img0008.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-Paraffin-NBP1-58268-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (AH54-10) [NBP2-67279] - Staining TPA in HepG2 cells (red). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/t-Plasminogen-Activator-tPA-Antibody-(AH54-10)-Immunocytochemistry-Immunofluorescence-NBP2-67279-img0004.jpg)
![Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody (AH54-10) [NBP2-67279] - Analysis of paraffin-embedded mouse liver tissue using anti-TPA Tubulin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/t-Plasminogen-Activator-tPA-Antibody-(AH54-10)-Immunohistochemistry-Paraffin-NBP2-67279-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (AH54-10) [NBP2-67279] - Staining TPA in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/t-Plasminogen-Activator-tPA-Antibody-(AH54-10)-Immunocytochemistry-Immunofluorescence-NBP2-67279-img0001.jpg)
![Western Blot: RNF130 Antibody [NBP1-81858] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-478](http://images.novusbio.com/images2/RNF130-Antibody-Western-Blot-NBP1-81858-img0006.jpg)
![Immunohistochemistry-Paraffin: RNF130 Antibody [NBP1-81858] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/images2/RNF130-Antibody-Immunohistochemistry-Paraffin-NBP1-81858-img0002.jpg)
![Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0019.jpg)
![Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.](http://images.novusbio.com/images2/Plasminogen-Antibody-Western-Blot-NB600-930-img0006.jpg)
![Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.](http://images.novusbio.com/images2/Plasminogen-Antibody-Western-Blot-NB600-930-img0005.jpg)
![SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00005555-P01-1.jpg)
