Von Willebrand Factor Antibody (2Q2134)

Western Blot: Von Willebrand Factor Antibody (2Q2134) [NBP1-39497] - Human placenta lysate was resolved by electrphoresis, transferred to PVDF membrane and probed with NBP1-39497 (1:1000). Proteins were visualized using and IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Arrow indicates protein von Willebrand Factor (310KD).

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody (2Q2134) [NBP1-39497] - Staining of human breast, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Product Discontinued

Von Willebrand Factor Antibody (2Q2134) Summary

• Immunogen: Purified human Factor VIII complex.
• Marker: Endothelial Cell Marker
• Specificity: Reacts strongly with human von Willebrand Factor or Factor VIII Related Antigen in both plasma and tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VWF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Radioimmunoassay 1:100-1:2000
  • Western Blot 1:1000
• Application Notes: Suitable for use in ELISA, RIA, Immunofluorescence, Western Blot (Detected vWF on 10ug of human placenta lysates.), Immunocytochemistry, Immunohistochemistry and Immunoperoxidase staining(Indirect Immunoperoxidase Staining: 1:50)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.6), 0.2% BSA and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 0.4 mg/ml
• Purity: Protein A purified

Alternate Names for Von Willebrand Factor Antibody (2Q2134)

• coagulation factor VIII VWF
• F8
• F8VWF
• von Willebrand factor
• VWD
• vWF

Background

Von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VWF
• Entrez:
  • 7450(Human)
• Uniprot:
  • P04275()