Von Hippel Lindau Antibody [DyLight 405]

Product Discontinued

Von Hippel Lindau Antibody [DyLight 405] Summary

• Immunogen: A synthetic peptide common to both human and mouse Von Hippel Lindau protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VHL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This Von Hippel Lindau antibody is useful for Immunoprecipitation (PMID 21658608) and Western blot. In Western blot a band is observed ~21kDa. The theoretical molecular weight of VHL is 24 kDa. However, different migrating species ranging from 21-30 kDa have been observed using antibodies to VHL, and may result from a variety of factors including alternatively spliced VHL mRNAs and protein degradation.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Von Hippel Lindau Antibody [DyLight 405]

• elongin binding protein
• HRCA1
• Protein G7
• pVHL
• RCA1
• VHL
• VHL1
• von Hippel-Lindau disease tumor suppressor
• von Hippel-Lindau syndrome
• von Hippel-Lindau tumor suppressor

Background

The von Hippel-Lindau (VHL) protein is a tumor suppressor that is highly conserved. VHL binds to transcription factor Elongin B and C subunits, and appears to prevent Elongin A from associating with the B and C subunits, thereby inhibiting the transcriptional activity of Elongin. The ability of VHL to block Elongin function may play a role in normal cell growth regulation. Mutated VHL sequences lose their ability to bind to Elongin subunits B and C, suggesting that the tumor suppression function of VHL may be linked to its ability to bind to Elongin. Likewise, the introduction of wild-type VHL suppresses growth in tumor cell lines lacking normal expression of VHL genes. The product of the VHL gene has multiple reported functions, the best characterized of which is its role as the recognition component of an ubiquitin E3 ligase complex responsible for mediating oxygen-dependent destruction of hypoxia-inducible factor-alpha (HIF-alpha) subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VHL