Von Hippel Lindau Antibody

Images

 
Western Blot: Von Hippel Lindau Antibody [NB100-485] - Detection of VHL in human kidney samples (30 ug)using NB 100-485. ECL detection 10 seconds. Lane 1: 0.5 ug/ml primary Lane 2: 2 ug/ml

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Von Hippel Lindau Antibody Summary

Immunogen
A synthetic peptide common to both human and mouse Von Hippel Lindau protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VHL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
This Von Hippel Lindau antibody is useful for Immunoprecipitation (PMID 21658608) and Western blot. In Western blot a band is observed ~21kDa. The theoretical molecular weight of VHL is 24 kDa. However, different migrating species ranging from 21-30 kDa have been observed using antibodies to VHL, and may result from a variety of factors including alternatively spliced VHL mRNAs and protein degradation. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-485 in the following applications:

Publications
Read Publications using
NB100-485 in the following applications:

  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Von Hippel Lindau Antibody

  • elongin binding protein
  • HRCA1
  • Protein G7
  • pVHL
  • RCA1
  • VHL1
  • von Hippel-Lindau disease tumor suppressor
  • von Hippel-Lindau syndrome
  • von Hippel-Lindau tumor suppressor

Background

The von Hippel-Lindau (VHL) protein is a tumor suppressor that is highly conserved. VHL binds to transcription factor Elongin B and C subunits, and appears to prevent Elongin A from associating with the B and C subunits, thereby inhibiting the transcriptional activity of Elongin. The ability of VHL to block Elongin function may play a role in normal cell growth regulation. Mutated VHL sequences lose their ability to bind to Elongin subunits B and C, suggesting that the tumor suppression function of VHL may be linked to its ability to bind to Elongin. Likewise, the introduction of wild-type VHL suppresses growth in tumor cell lines lacking normal expression of VHL genes. The product of the VHL gene has multiple reported functions, the best characterized of which is its role as the recognition component of an ubiquitin E3 ligase complex responsible for mediating oxygen-dependent destruction of hypoxia-inducible factor-alpha (HIF-alpha) subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Von Hippel Lindau Antibody (NB100-485)(4)

Review for Von Hippel Lindau Antibody (NB100-485) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-485:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 08/10/2010
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Summary

ApplicationWestern Blot
Sample Tested293T Cell Lysate, Sample Amount: 50 ug
SpeciesHuman
CommentsEndogenous VHL from 293T cells detected.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:400, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Comments

CommentsEndogenous VHL from 293T cells detected.

Product General Protocols

View specific protocols for Von Hippel Lindau Antibody (NB100-485): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Von Hippel Lindau Antibody (NB100-485). (Showing 1 - 1 of 1 FAQs).

  1. I'm not sure that this Ab can distinghish between VHL wild-type and the VHL mutated form that is present in renal cell carcinoma patients. Do you have some evidence that it can?
    • We have not actually tested if this antibody can distinguish between the two forms.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-485AF405
Alexa Fluor 488 Labeled NB100-485AF488
Alexa Fluor 647 Labeled NB100-485AF647
Alexa Fluor 700 Labeled NB100-485AF700
Biotin Labeled NB100-485B
DyLight 405 Labeled NB100-485V
DyLight 488 Labeled NB100-485G
DyLight 550 Labeled NB100-485R
DyLight 650 Labeled NB100-485C
HRP Labeled NB100-485H

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Recent Reviews

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0
4
1
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2
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1
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Anonymous
08/10/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol VHL
Entrez
Uniprot