Von Hippel Lindau Antibody (NB100-1899)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Von Hippel Lindau Antibody Summary

Immunogen
A synthetic peptide derived from the human Von Hippel Lindau protein. [UniProt# P40337]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VHL
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This Von Hippel Lindau antibody is useful in Western blot, Immunohistochemistry-paraffin embedded sections and Immunoprecipitation. In Western blot bands are observed ~19 and 30 kDa. The theoretical molecular weight of VHL is 24 kDa. However, different migrating species ranging from 21-30 kDa have been observed using antibodies to VHL, and may result from a variety of factors including alternatively spliced VHL mRNAs and protein degradation. This antibody is also useful in Immunoprecipitation (PMID: 21658608) and Immunohistochemistry-paraffin embedded sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-1899 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human. Not expected to work in mouse based on sequence divergence.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Von Hippel Lindau Antibody

  • elongin binding protein
  • HRCA1
  • Protein G7
  • pVHL
  • RCA1
  • VHL1
  • von Hippel-Lindau disease tumor suppressor
  • von Hippel-Lindau syndrome
  • von Hippel-Lindau tumor suppressor

Background

The von Hippel-Lindau (VHL) protein is a tumor suppressor that is highly conserved. VHL binds to transcription factor Elongin B and C subunits, and appears to prevent Elongin A from associating with the B and C subunits, thereby inhibiting the transcriptional activity of Elongin. The ability of VHL to block Elongin function may play a role in normal cell growth regulation. Mutated VHL sequences lose their ability to bind to Elongin subunits B and C, suggesting that the tumor suppression function of VHL may be linked to its ability to bind to Elongin. Likewise, the introduction of wild-type VHL suppresses growth in tumor cell lines lacking normal expression of VHL genes. The product of the VHL gene has multiple reported functions, the best characterized of which is its role as the recognition component of an ubiquitin E3 ligase complex responsible for mediating oxygen-dependent destruction of hypoxia-inducible factor-alpha (HIF-alpha) subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Von Hippel Lindau Antibody (NB100-1899)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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(2)
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WB Video Protocol

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Bioinformatics

Gene Symbol VHL
Entrez
Uniprot