This Von Hippel Lindau antibody is useful in Western blot, Immunohistochemistry-paraffin embedded sections and Immunoprecipitation. In Western blot bands are observed ~19 and 30 kDa. The theoretical molecular weight of VHL is 24 kDa. However, different migrating species ranging from 21-30 kDa have been observed using antibodies to VHL, and may result from a variety of factors including alternatively spliced VHL mRNAs and protein degradation. This antibody is also useful in Immunoprecipitation (PMID: 21658608) and Immunohistochemistry-paraffin embedded sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-1899 in the following applications:
Human. Not expected to work in mouse based on sequence divergence.
Alternate Names for Von Hippel Lindau Antibody
elongin binding protein
von Hippel-Lindau disease tumor suppressor
von Hippel-Lindau syndrome
von Hippel-Lindau tumor suppressor
The von Hippel-Lindau (VHL) protein is a tumor suppressor that is highly conserved. VHL binds to transcription factor Elongin B and C subunits, and appears to prevent Elongin A from associating with the B and C subunits, thereby inhibiting the transcriptional activity of Elongin. The ability of VHL to block Elongin function may play a role in normal cell growth regulation. Mutated VHL sequences lose their ability to bind to Elongin subunits B and C, suggesting that the tumor suppression function of VHL may be linked to its ability to bind to Elongin. Likewise, the introduction of wild-type VHL suppresses growth in tumor cell lines lacking normal expression of VHL genes. The product of the VHL gene has multiple reported functions, the best characterized of which is its role as the recognition component of an ubiquitin E3 ligase complex responsible for mediating oxygen-dependent destruction of hypoxia-inducible factor-alpha (HIF-alpha) subunits.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.