Von Hippel Lindau Antibody

Product Discontinued

Von Hippel Lindau Antibody Summary

• Immunogen: A synthetic peptide derived from the human Von Hippel Lindau protein. [UniProt# P40337]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VHL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: This Von Hippel Lindau antibody is useful in Western blot, Immunohistochemistry-paraffin embedded sections and Immunoprecipitation. In Western blot bands are observed ~19 and 30 kDa. The theoretical molecular weight of VHL is 24 kDa. However, different migrating species ranging from 21-30 kDa have been observed using antibodies to VHL, and may result from a variety of factors including alternatively spliced VHL mRNAs and protein degradation. This antibody is also useful in Immunoprecipitation (PMID: 21658608) and Immunohistochemistry-paraffin embedded sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Not expected to work in mouse based on sequence divergence.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Von Hippel Lindau Antibody

• elongin binding protein
• HRCA1
• Protein G7
• pVHL
• RCA1
• VHL
• VHL1
• von Hippel-Lindau disease tumor suppressor
• von Hippel-Lindau syndrome
• von Hippel-Lindau tumor suppressor

Background

The von Hippel-Lindau (VHL) protein is a tumor suppressor that is highly conserved. VHL binds to transcription factor Elongin B and C subunits, and appears to prevent Elongin A from associating with the B and C subunits, thereby inhibiting the transcriptional activity of Elongin. The ability of VHL to block Elongin function may play a role in normal cell growth regulation. Mutated VHL sequences lose their ability to bind to Elongin subunits B and C, suggesting that the tumor suppression function of VHL may be linked to its ability to bind to Elongin. Likewise, the introduction of wild-type VHL suppresses growth in tumor cell lines lacking normal expression of VHL genes. The product of the VHL gene has multiple reported functions, the best characterized of which is its role as the recognition component of an ubiquitin E3 ligase complex responsible for mediating oxygen-dependent destruction of hypoxia-inducible factor-alpha (HIF-alpha) subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Von Hippel Lindau Antibody (NB100-1899)(3)

Publications using NB100-1899

• Ma CN, Wo LL, Wang DF, et al. Hypoxia activated long non-coding RNA HABON regulates the growth and proliferation of hepatocarcinoma cells by binding to and antagonizing HIF-1 alpha RNA biology 2021-01-21 [PMID: 33478328] (WB, Human)
• Xi H, Gao YH, Han DY et al. Hypoxia inducible factor-1a suppresses Peroxiredoxin 3 expression to promote proliferation of CCRCC cells. FEBS Lett. 2014-08-02 [PMID: 25093297] (WB, Human)
• Esteban, MA et al. Regulation of E-cadherin expression by VHL hypoxia-inducible factor. Cancer Res 66(7): 3567-3575. 2006-01-01 [PMID: 16585181] (WB, Human)

Bioinformatics

• Gene Symbol: VHL
• Entrez:
  • 7428(Human)
• Uniprot:
  • P40337(Human)