Vitamin D BP Antibody [Unconjugated]

Western blot shows lysates of mouse liver. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vitamin D BP at approximately 52-58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Vitamin D BP at approximately 58 kDa (as indicated) using 5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Vitamin D BP Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Vitamin D BP
  Leu17-Ser476
  Accession # P21614
• Specificity: Detects mouse Vitamin D BP in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Vitamin D BP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vitamin D BP Antibody [Unconjugated]

• DBP
• DBP/GC
• DBP-MAF
• DBPVDBG
• EC 6.3.1.5
• Gc
• gc-globulin
• Gc-MAF
• GRD3
• group-specific component (vitamin D binding protein)
• Group-specific component
• hDBP
• VDB
• VDBG
• VDBP
• Vitamin D BP
• vitamin D-binding alpha-globulin
• vitamin D-binding protein

Background

VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52‑58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26‑476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346‑421, a second shows a 67 aa substitution for aa 345‑421, and a third shows a 34 aa substitution for aa 346‑423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17‑476) is 77% and 90% aa identical to human and rat VDBP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vitamin D BP Antibody (AF4188)(2)

Publications using AF4188

• Eijken, M;Krautzberger, AM;Scholze-Wittler, M;Boers-Sijmons, B;Koedam, M;Kosiol, B;Schrewe, H;van Leeuwen, JP;van der Eerden, BC; Vasorin-deficient mice display disturbed vitamin D and mineral homeostasis in combination with a low bone mass phenotype Bone reports 2024-09-01 [PMID: 39157725] (ELISA Capture, Mouse)
• S Dhital, NR Vyavahare Nanoparticle-based targeted delivery of pentagalloyl glucose reverses elastase-induced abdominal aortic aneurysm and restores aorta to the healthy state in mice PLoS ONE, 2020-03-27;15(3):e0227165. 2020-03-27 [PMID: 32218565] (IHC, Mouse)

Bioinformatics

• Gene Symbol: GC
• Uniprot:
  • P21614()