Vitamin D BP Antibodies

HAF016). A specific band was detected for Vitamin D BP at approximately 52-58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for Vitamin D BP at approximately 52-58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse liver. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Vitamin D BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4188) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vitamin D BP at approximately 52-58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

MAB41881) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB41881) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Vitamin D BP protein was serially diluted 2-fold and captured by Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB41881) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Vitamin D BP protein was serially diluted 2-fold and captured by Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB41881) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />