Villin 1 Antibody (3E5G11) - BSA Free

Western Blot: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis using VIL1 mouse mAb against SW620 cell lysate.

Immunocytochemistry/Immunofluorescence: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis of Hela cells using VIL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry-Paraffin: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis of paraffin-embedded cervical cancer tissues using VIL1 mouse mAb with DAB staining.

Flow Cytometry: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis of SW620 cells using VIL1 mouse mAb (green) and negative control (red).

Western Blot: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis using VIL1 mAb against HEK293 (1) and VIL1 (AA: 1-209)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: Villin 1 Antibody (3E5G11) [NBP2-52585] - Analysis using VIL1 mAb against human VIL1 (AA: 1-209) recombinant protein. (Expected MW is 49.4 kDa)

ELISA: Villin 1 Antibody (3E5G11) [NBP2-52585] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 3E5G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Villin 1 Antibody (3E5G11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Villin 1 (AA: 1-209) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VIL1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 92.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Villin 1 Antibody (3E5G11) - BSA Free

• D2S1471
• VIL
• villin 1
• villin-1

Background

Villin 1 encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VIL1