VIAAT/SLC32A1/VGAT Antibody

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, MiAr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
4.3 mg/ml

Order Details

VIAAT/SLC32A1/VGAT Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of mouse VGAT.
Localization
Integral membrane protein. Intracellular membrane vesicles
Specificity
VGAT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC32A1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • ELISA
  • Microarray
Application Notes
Use in ELISA reported in scientific literature (PMID 24772072)
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB110-55238 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Rat, Mouse. This sequence is identical in rat VGAT and highly conserved (single a.a. substitution) in human VGAT.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
4.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VIAAT/SLC32A1/VGAT Antibody

  • bA122O1.1
  • hVIAAT
  • SLC32A1
  • solute carrier family 32 (GABA vesicular transporter), member 1
  • Solute carrier family 32 member 1
  • Vesicular GABA transporter
  • vesicular inhibitory amino acid transporter
  • VGAT
  • VGATGABA and glycine transporter
  • VIAAT

Background

Synaptic transmission involves the regulated exocytosis of vesicles containing neurotransmitters. The inhibitory neurotransmitter g-aminobutyric acid (GABA) is synthesized in the cytoplasm from L-glutamate by the enzyme glutamic acid decarboxylase (GAD) and loaded in synaptic vesicles for exocytosis. The vesicular GABA transporter (VGAT, also termed vesicular inhibitory amino acid transporter or VIAAT, 52-57 kDa) is responsible for the uptake of GABA into synaptic vesicles, following synthesis by GAD, or reuptake by plasma membrane GABA transporters (GATs). VGAT protein (55 kDa) contains ten transmembrane domains and belongs to a new family of vesicular neurotransmitter transporters. VGAT is highly conserved in mouse, rat and human (95-98% identity). VGAT uses both chemical and electrical components of the luminal protein gradient to mediate the accumulation of GABA into synaptic vesicles. VGAT is also responsible for glycine uptake in glycinergic neurons. VGAT is a homolog of the c. elegans unc -47 protein involved in GABA transport. Unc-47 is expressed in GABA neurons, localizes to synaptic vesicles and confers vesicular GABA transport in transfected cells. Mutants of c. elegans in which the unc-47 gene encoding VGAT is non-functional or absent, exhibit a complete loss of GABAergic function and elevated levels of GABA in the cytoplasm. VGAT is present in nerve endings of inhibitory neurons containing GABA, but also in glycinergic neurons in the brain and retina.1,4,5 At subcellular levels, VGAT specifically associates with synaptic vesicles. VGAT/VIAAT is also expressed in rat pancreatic islet cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VIAAT/SLC32A1/VGAT Antibody (NB110-55238)(3)

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Diseases for VIAAT/SLC32A1/VGAT Antibody (NB110-55238)

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Bioinformatics

Gene Symbol SLC32A1
Entrez
Uniprot