VGLUT1 Antibody (CL2754) - Azide and BSA Free

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue

Analysis in human cerebral cortex and tonsil tissues using NBP3-43811 antibody. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.

Staining of human cerebral cortex shows strong positivity in neuropil.

Staining of human tonsil shows no positivity in lymphoid cells as expected.

Staining of mouse brain shows strong positivity in glutamatergic fibers in the molecular and granular layers of the cerebellum.

Staining of rat brain shows strong positivity in glutamatergic fibers in the polymorph layer of dentate gyrus.

Staining of rat brain shows strong positivity in glutamatergic processes in the molecular and granular layers of cerebellum.

Staining of mouse hippocampus shows strong positivity in glutamatergic fibers in the CA2 layer.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL2754
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VGLUT1 Antibody (CL2754) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse VGLUT1 Antibody (CL2754) - Azide and BSA Free (NBP2-46627) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9P2U7, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC17A7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for VGLUT1 Antibody (CL2754) - Azide and BSA Free

• BNPI
• BNPIbrain-specific Na-dependent inorganic phosphate cotransporter
• Brain-specific Na(+)-dependent inorganic phosphate cotransporter
• member 7
• SLC17A7
• solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter)
• Solute carrier family 17 member 7
• VGLUT1
• VGLUT1vesicular glutamate transporter 1

Background

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC17A7