Versican Antibody (255915)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 255915
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Versican Antibody (255915) Summary

• Immunogen: E. coli-derived recombinant human Versican isoform V0
  Gly1344-Asp1554
  Accession # P13611
• Specificity: Detects human Versican in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: VCAN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Versican Antibody (255915)

• Chondroitin sulfate proteoglycan 2WGN
• Chondroitin sulfate proteoglycan core protein 2
• CSPG2
• CSPG2DKFZp686K06110
• ERVR
• GHAP
• Glial hyaluronate-binding protein
• Large fibroblast proteoglycan
• PG-M
• PG-MWGN1
• VCAN
• versican core protein
• versican proteoglycan
• Versican

Background

Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Versican Antibody (MAB3054)(2)

Publications using MAB3054

• Sotoodehnejadnematalahi F, Staples K, Chrysanthou E, Pearson H, Ziegler-Heitbrock L, Burke B Mechanisms of Hypoxic Up-Regulation of Versican Gene Expression in Macrophages. PLoS ONE, 2015-06-09;10(6):e0125799. 2015-06-09 [PMID: 26057378] (Flow Cytometry, Human)
• Singh A, Satchell SC, Neal CR Glomerular endothelial glycocalyx constitutes a barrier to protein permeability. J. Am. Soc. Nephrol., 2007-10-17;18(11):2885-93. 2007-10-17 [PMID: 17942961] (Western Blot, Human)

Bioinformatics

• Gene Symbol: VCAN
• Uniprot:
  • P13611()