VEGFR2/KDR/Flk-1 Antibody

Product Discontinued

VEGFR2/KDR/Flk-1 Antibody Summary

• Immunogen: Residues corresponding to the kinase insert or C-terminus of mouse VEGF Receptor 2 (residues 1158-1365). [Swiss-Prot# P35918]
• Marker: Endothelial Cell Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDR
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ul
  • Western Blot 1:500
• Application Notes: This VEGF Receptor 2 antibody is useful for IHC-P sections (PMID 10751359), Immunoprecipitation and WB where a doublet can been seen at ~ 150 kDa.
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for VEGFR2/KDR/Flk-1 Antibody

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

VEGFs such as VEGFA, VEGFC, VEGFD etc. activate RTKs namely VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4), that regulate angiogenic as well as lymphangiogenic transduction signals. VEGF Receptor 2 (VEGFR2) is the most prominent cell-surface receptor for VEGF ligands, and binding of VEGFs to isoform 1 leads to multiple signaling cascade activations. PLCG1 activation results in production of signaling molecules (diacylglycerol, inositol 1,4,5-trisphosphate etc) and PKC activation. VEGFR2 is involved in the activation of MAPK1/ERK2, MAPK3/ERK1, and MAPK as well as AKT signaling pathways. It facilitates phosphorylation of PIK3R1 (regulatory subunit of PI3K), cytoskeletal actin reorganization and PTK2/FAK1 activation. VEGFR2 is essential for VEGFA-mediated induction of NOS2/NOS3, leading to the production of NO by endothelial cells and is involved in phosphorylation of FYN, NCK1, NOS3, PIK3R1 and SRC. Isoforms -2 and -3 function as decoy receptors for VEGFs, and isoform -2 plays as negative regulator of VEGFA/VEGFC-mediated lymphangiogenesis by limiting free VEGFA and/or VEGFC levels alongwith preventing their binding to VEGFR3. Versatile signaling effects by VEGFR2 leads to its regulatory role in processes such as angiogenesis, vascular development, vascular permeability, embryonic hematopoiesis, and promotes proliferation, survival, migration and differentiation of endothelial cells alongwith facilitating reorganization of the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR2/KDR/Flk-1 Antibody (NB100-627)(11)

Publications using NB100-627

• Fittipaldi R, Floris P, Proserpio V et al. The Lysine Methylase SMYD3 Modulates Mesendodermal Commitment during Development Cells 2021-05-18 [PMID: 34069776] (WB, Human)
• Gevariya N, Lachance G, Robitaille K, et al. Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity Molecular cancer research : MCR 2020-12-01 [PMID: 33262291] (WB, Human)
• Podemska-Jedrzejczak Z, Malinska A, et al. Vascular restenosis in coronary artery bypass grafting might be associated with VEGF-C/VEGFR-3 signaling pathway. Heart Vessels 2018-09-01 [PMID: 29557990] (IF/IHC, Human)
• Ying R, Wang XQ, Yang Y et al. Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway. Life Sci. 2016-01-01 [PMID: 26656263] (WB, Human)
• Andre H, Tunik S, Aronsson M, Kvanta A. Hypoxia-Inducible Factor-1alpha Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model. ARVO Journals. 2015-10-01 [PMID: 26501235] (WB, Mouse)
• Tsuboi I, Yamashita T, Nagano M et al. Impaired expression of HIF -2alpha induces compensatory expression of HIF-1alpha for the recovery from anemia J. Cell. Physiol. 2015-01-03 [PMID: 25557133] (WB, Mouse)
• Puglisi F, Puppin C, Pegolo E et al. Expression of periostin in human breast cancer. J Clin Pathol;614:494-8. 2008-04-01 [PMID: 17938160]
• Singh, AJ et al. The carboxyl terminus of VEGFR-2 is required for PKC-mediated down-regulation. Mol Biol Cell;164:2106-18. 2005-04-01 [PMID: 15673613] (IP, WB, Mouse)
• Meyer, RD et al. The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling. J Biol Chem;2791:735-42. 2004-01-02 [PMID: 14573614] (IP, WB)
• Lashkari K, Hirose T, Yazdany J, McMeel JW, Kazlauskas A, Rahimi N. Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity. Am J Pathol;1564:1337-44. 2000-04-01 [PMID: 10751359] (IHC-P, Human)
• Rahimi, N et al. Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 VEGFR-1 modulates mitogenic activity of VEGFR-2 in endothelial cells. J Biol Chem;27522:16986-92. 2000-06-02 [PMID: 10747927] (IP, WB, Mouse)

Reviews for VEGFR2/KDR/Flk-1 Antibody (NB100-627) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Mouse, Sheep.

reviewed by: GAIL SILVER WB Sheep 05/16/2017

Summary

• Application: Western Blot
• Sample Tested: caruncle
• Species: Sheep
• Lot: A2

reviewed by: Valentina Villani WB Mouse 01/17/2017

Summary

• Application: Western Blot
• Sample Tested: mouse renal endothelial cells
• Species: Mouse

reviewed by: Verified Customer WB Mouse 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: GL261 implanted mouse glioma tissue
• Species: Mouse
• Lot: A

FAQs for VEGFR2/KDR/Flk-1 Antibody (NB100-627). (Showing 1 - 1 of 1 FAQs).

1. Does VEGFR-2 antibody show positive staining in cytoplasm or membrane/nuclear?
   • The VEGF R2 protein can be detected in several locations inside of a cell. For the most helpful information, please see the following link to the Uniprot entry for this protein: http://www.uniprot.org/uniprot/P35918#subcellular_location. Our antibody is made to a sequence between aa 1158-1365 of the human protein. This portion is conserved in isoform 1 of the VEGF R2 protein and should not detect isoform 2. Depending on your samples, the VEGF R2 protein may localize to different areas within the cell. I would recommend checking publications that have tested for this protein in similar samples for expected results.

Bioinformatics

• Gene Symbol: KDR
• Entrez:
  • 3791(Human)
  • 16542(Mouse)
• Uniprot:
  • P35918()