Mouse VEGF-C ELISA Kit (Colorimetric)

ELISA: Mouse VEGF-C ELISA Kit (Colorimetric) [NBP2-78893] - Samples were spiked with high concentrations of Mouse VEGF-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse VEGF-C ELISA Kit (Colorimetric) [NBP2-78893] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, and other biological fluids.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL

Mouse VEGF-C ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse VEGF-C in samples. No Significant cross-reactivity or interference between Mouse VEGF-C and analogues was observed.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.09%
• Intra-Assay: CV% < 4.73%
• Spike Recovery: 90-107%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VEGFC

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse VEGF-C ELISA Kit (Colorimetric)

• Flt4 ligand
• Flt4-L
• vascular endothelial growth factor C
• Vascular endothelial growth factor-related protein
• VEGFC
• VEGF-C
• VRPFLT4 ligand DHM

Background

Vascular endothelial growth factors (VEGFs), also known as vasculotropins, are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. The target cell specificity of VEGF is restricted to vascular endothelial cells. Vascular Endothelial Growth Factor C (VEGFC) is a member of the VEGF subfamily of PDGF-related growth factors. It is the ligand for Flt4 (VEGFR3) and KDR (VEGFR2). VEGFC binds Flt4 and induces tyrosine autophosphorylation of VEGFR3 and VEGFR2. VEGFC also stimulates the migration of bovine capillary endothelial cells in collagen gel. It is a specific growth factor for the lymphatic vascular system and mediates lymphangiogenesis. VEGFC is abundantly expressed in heart and skeletal muscle. Other tissues such as lung and kidney also express VEGFC. The human VEGFC gene is located on chromosome 4q34.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for VEGF-C ELISA Kit (NBP2-78893)(4)

Publications using NBP2-78893

• Lu R, Kolarzyk A, Stamer W et al. Human ocular fluid outflow on-chip reveals trabecular meshwork-mediated Schlemm’s canal endothelial dysfunction in steroid-induced glaucoma Nature cardiovascular research 2025-08-27 [PMID: 40866626]
• Justin Vercellino, Beata Małachowska, Shilpa Kulkarni, Brett I. Bell, Shahin Shajahan, Kosaku Shinoda, Gary Eichenbaum, Amit K. Verma, Sanchita P. Ghosh, Weng-Lang Yang, Paul S. Frenette, Chandan Guha Thrombopoietin mimetic stimulates bone marrow vascular and stromal niches to mitigate acute radiation syndrome Stem Cell Research & Therapy 2024-04-29 [PMID: 38679747]
• Feng W, Jiang D, Xu Y et al. CDK4/6i enhances the antitumor effect of PD1 antibody by promoting TLS formation in ovarian cancer Heliyon 2023-09-01 [PMID: 37809574] (ELISA)
• yamada H, Naito R, Nishimura M et al. Xenotransplantation of neonatal porcine bone marrow-derived mesenchymal stem cells improves diabetic wound healing by promoting angiogenesis and lymphangiogenesis Xenotransplantation 2022-03-12 [PMID: 35279886]

Review for VEGF-C ELISA Kit (NBP2-78893) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 07/23/2020

Summary

• Sample Tested: bEnd.3 mouse endothelioma cell line
• Lot: 168566000

FAQs for VEGF-C ELISA Kit (NBP2-78893). (Showing 1 - 2 of 2 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VEGFC
• Uniprot:
  • P97953(Mouse)