HAF017). Specific bands were detected for VEGF-C at approximately 52 kDa, 34 kDa, and 13 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">

HAF017). Specific bands were detected for VEGF-C at approximately 52 kDa, 34 kDa, and 13 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (
HAF017). Specific bands were detected for VEGF-C at approximately 52 kDa, 34 kDa, and 13 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in crypts of the colon mucusa (longitudinal section of crypts). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for
Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in crypts of the colon mucusa (longitudinal section of crypts). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for
Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
MAB752) coated on a Clear Polystyrene Microplate (
DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." class="big_lightbox">

MAB752) coated on a Clear Polystyrene Microplate (
DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." class="big_thumb" />
752-VC) was serially diluted and captured by Mouse Anti-Human VEGF‑C Monoclonal Antibody (Catalog #
MAB752) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

752-VC) was serially diluted and captured by Mouse Anti-Human VEGF‑C Monoclonal Antibody (Catalog #
MAB752) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />