Rat VEGF-C ELISA Kit (Colorimetric)

ELISA: VEGF-C ELISA Kit (Colorimetric) [NBP1-92698]

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (heparin)
• Standard Curve Range: 47.0 - 3,000 pg/mL
• Sensitivity: 27.0 pg/mL

Rat VEGF-C ELISA Kit (Colorimetric) Summary

• Description: The rat VEGF-C ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat VEGF-C
• Specificity: The assay detects both natural and recombinant rat VEGF-C. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat VEGF-C positive serum. There was no cross reactivity or interference detected, notably not with rat VEGF-A, VEGF-B, VEGF-D, PDGF-AA,PDGF-BB, PLGF, HGF, EGF, VEGF-R1 and KDR.
• Standard Curve Range: 47.0 - 3,000 pg/mL
• Sensitivity: 27.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 2.7%
• Intra-Assay: CV% < 5.1%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VEGFC

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat VEGF-C ELISA Kit (Colorimetric)

• Flt4 ligand
• Flt4-L
• vascular endothelial growth factor C
• Vascular endothelial growth factor-related protein
• VEGFC
• VEGF-C
• VRPFLT4 ligand DHM

Background

Vascular endothelial growth factor-C (VEGF-C) is a member of the VEGF family of cytokines. It has been shown to exhibit angiogenic and lymphangiogenic actions. The VEGF family of growth factors and receptors is involved in the development and growth of the vascular endothelial system. Two of its family members, VEGF-C and VEGF-D, regulate the lymphatic endothelial cells via their receptor VEGFR3, thus acting as mitogens for these cells. VEGF-C expression is associated with hematological malignancies. Like VEGF it acts as survival factor on leukemia. Together with the expression of their receptors, VEGF and VEGF-C result in the generation of autocrine loops that may support cancer cell survival and proliferation. Further VEGF-C expression has been shown in gastrointestinal tract malignancies where it correlates with lymphatic invasion, lymphnode metastasis and reduced survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VEGF-C ELISA Kit (NBP1-92698). (Showing 1 - 2 of 2 FAQ).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VEGFC